It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

為了研發(fā)預(yù)防副結(jié)核病的新型疫苗尤其是DNA疫苗及相關(guān)蛋白功能国产一级在视频，本研究選擇了MAP的主要保護(hù)性抗原Hsp65蛋白老人把我添到了高潮A片，以副結(jié)核分枝桿菌C-2株染色體DNA為模板久久黃片，以hsp65基因的特異性引物進(jìn)行PCR擴(kuò)增无码有码日韩制服AV，獲得了1 626bp的hsp65基因，通過T-A克隆技術(shù)99热精品2，將PCR產(chǎn)物克隆至pGEM-T Vector中91小视频国产在线，以質(zhì)粒大小、酶切分析美女超污视频网站、PCR擴(kuò)增及序列分析鑒定重組克隆日韩一级欧美一级高清片w，成功地構(gòu)建出克隆質(zhì)粒pGEM-T-hsp65，以DNASTAR軟件分析了所克隆基因的核苷酸序列和推導(dǎo)的氨基酸序列香蕉亚洲91一本大道，結(jié)果表明在线亚洲国产鲁一鲁网，所獲得的hsp65基因與GenBank中MAPK-10株該基因核苷酸大小完全一致，兩者核苷酸序列的同源性為99.1％91色色福利视频，氨基酸序列的同源性為99.3％在线观看视频免费网站黄，表明該基因在副結(jié)核分枝桿菌中高度保守。

Another two field container experiments were conducted to study intraclonal spatial division of labour in Fragaria vesca and Lysimachia christinae with different branching types and Lysimachia christinae at different altitudes in response to reciprocal patchiness of light and nutrients.

結(jié)果顯示：克隆整合顯著增強(qiáng)蛇莓和野草莓脅迫分株的生長欧美性色欧美A在线观看，耗-益分析表明對未受脅迫分株沒有顯著損耗特黄一级乱淫A片，故整個(gè)克隆片段的生長得到顯著提高，克隆整合對于克隆植物在高山環(huán)境中的種群擴(kuò)展三级片在线无码、生境開拓可能是非常重要的久久免费网址。

The cowpea number per pod, weight per pod, length per pod, number of inflorescence; and the height of main stem, number of branches and node of main stem at seedling-pulling at each single plant were investigated for the yield-calculating, and meanwhile, the analysis of relevant factor and path analysis of the component of yield/plant was conducted.

方法］定株調(diào)查每株美國無架豇豆的豆英數(shù)、英重日韩中文福利片观看、英長最新中文字幕在线视频网站、花序數(shù)，拉秧時(shí)測定主莖高a黄大片久久网、分枝數(shù)及主莖節(jié)數(shù)在线观看中文字幕网，并計(jì)算單株產(chǎn)量，進(jìn)行單株產(chǎn)量構(gòu)成因素的相關(guān)分析和通徑分析日韩女神精品自拍视频。

Finally,the WISCONSIN Sequence Analysis Package (GCG,10.2 version),Phylogenetic Analysis Using Parsimony (PAUP,4.0 version) and Treeview (1.5 version) software were used for data analysis,sequence translation,phylogenetic tree drawing and molecular evolution analysis.

本研究結(jié)果證明天天视频在线50页免费观看，H9N2亞型人體分離株屬于G1-like亞分支的G1-HK小分支，該小分支毒株之間具有很高的同源性黄色网站在线免费观看视频大全，因此該小分支AIV可能具有感染人的可能性yiyinav，是具有引起人流感暴發(fā)潛能的病毒免费欧美黄色。

By using cluster analysis, the tested cultivars are classified into two groups (bush vineⅠand semi-bush vine Ⅱ) and four sub-groups (Ⅰ1,Ⅰ2,Ⅱ1 and Ⅱ2). Plant type has a more important effect on C. pepo yield formation.

結(jié)果表明：反映西葫蘆株型的主要指標(biāo)是株高一级在线高清全视频、莖節(jié)長、平均葉面積、功能葉片數(shù)和葉片傾角日婬片A片AAA毛片在线；通過聚類分析把參試品種分為兩類4個(gè)亞類好大好爽要高潮了视频，即矮蔓Ⅰ（Ⅰ1和Ⅰ2）和半矮蔓Ⅱ（Ⅱ1和Ⅱ2）。

The nitrate non-utilizing mutantas an another added genetic marker was used to select the out-crossed perithecium from self-crossed perithecia. Seven crosses were tested in all, including cross between S×S, MR ×S, MR×MR,HR ×S, as well as HR ×MR. The results showed that no recombinant phenotype was found among all progeny of seven crosses. Progeny segregation of crosses between the parents with different sensitive levels (i.e. MR×S, HR×S,HR×MR) clearly fit a 1:1 ratio of two parental phenotypes, and no segregation was found in the crosses of S×S and MR × MR.

從25個(gè)敏感菌株和31個(gè)抗性菌株中隨機(jī)挑選了2個(gè)S免费A片国产毛无码A片久久、3個(gè)MR和1個(gè)HR偷拍日韩无码一区，并以硝酸鹽營養(yǎng)缺陷型突變體作為另一個(gè)遺傳標(biāo)記，按照S×S亚洲熟女性爱视频、MR×MR男女av网站有那些、MR×S、HR×S一级片免费视频精品、HR×MR等共設(shè)計(jì)了7個(gè)雜交組合A片无网，對各雜交后代對多菌靈的敏感性測試發(fā)現(xiàn)，在所有雜交后代中均未出現(xiàn)除雙親表現(xiàn)型以外的重組型個(gè)體黄色网站免费一级国产性生活破处真人版，MR×S成年男性网站、HR×S及HR×MR的雜交后代出現(xiàn)了1∶1的分離比例。

Nature sent him into the world strong and lusty, in a thriving condition, wearing his own hair on his head, the proper branches of this reasoning vegetable, till the axe of intemperance has lopped off his green boughs, and left him a withered trunk, he then flies m art, and puts on a periwig, valuing himself upon an unnatural bundle of baits, all covered with powder, that never grew on his head, but now should this our broomstick pretend to enter the scene, proud of those birchen spoils it never bore, and all coveted with dust,through the sweepings of the finest lady's chamber, we should be apt to ridicule and despise its vanity Partial judges that we are of our own excellencies, and other men's defaults!

當(dāng)大自然送他入世之初性激慰在线网站观看，他是強(qiáng)壯有力的变态欧美亚洲日韩女优电影在线观看，處于興旺時(shí)期，滿頭的天生好發(fā)日本高清www在线观看视频；如果比作一株有理性的植物一级A片免费黄片，那就是枝葉齊全。但不久酗酒貪色就像一把斧子砍掉了他的青枝綠葉永久精品在线观看视频，只留給他一根枯株看外国免费免下载男女人与抽黄色视频。他趕緊求助于人工，戴上了頭套啦啦啦资源在线观看视频4，以一束撲滿香粉但非他頭上所長的假發(fā)為榮无码A片在线免费看片。要是我們這把掃帚也這樣登場，由于把一些別的樹條收集到身上而得意洋洋又粗又黑又长欧美视频，其實(shí)這些條上盡是塵土性生活免费播放视频欧美，即使是最高貴的夫人房里的塵土，我們一定會笑它如何虛榮吧黄片一男一女免费！我們就是這樣偏心的審判官黄色网站永久视频，偏于自己的優(yōu)點(diǎn)，別人的毛踩蘸放访涝谙摺69久久夜色精品国产69–！1.pretend："自認(rèn)為有能力"的意思，根據(jù)上下文的情況可以翻譯為"想"

Nature sent him into the world strong and lusty, in a thriving condition, wearing his own hair on his head, the proper branches of this reasoning vegetable, till the axe of intemperance has lopped off his green boughs, and left him a withered trunk; he then flies to art, and puts on a periwig, valuing himself upon an unnatural bundle of hairs, all covered with powder, that never grew on his head; but now should this our broomstick pretend to enter the scene, proud of those birchen spoils it never bore, and all covered with dust, through the sweepings of the finest lady's chamber, we should be apt to ridicule and despise its vanity.

當(dāng)大自然送他入世之初黄色网站在线看的黄色网站，他是強(qiáng)壯有力的91精品国产综合久久精品图片，處于興旺時(shí)期午夜丰满少妇拍拍视频，滿頭的天生好發(fā)；如果比作一株有理性的植物人人操乱搞，那就是枝葉齊全黄色外国男人叉外国女人日逼视频。但不久酗酒貪色就像一把斧子砍掉了他的青枝綠葉，只留給他一根枯株日韩AⅤ大全网址。他趕緊求助于人工精品偷拍43p，戴上了頭套，以一束撲滿香粉但非他頭上所長的假發(fā)為榮欧美曰韩视频在线一区。要是我們這把掃帚也這樣登場黄色靓女网站，由于把一些別的樹條收集到身上而得意洋洋，其實(shí)這些條上盡是塵土中国jizzz，即使是最高貴的夫人房里的塵土亚洲第一区二区在线观看，我們一定會笑它是如何虛榮吧！

The baumanii resistance shows upgrade tendency overall, and the persister increases rapidly as well as multi-drug resistance because of widely use even abuse of antibiotics.

由于抗菌藥物的廣泛使用甚至過度使用国产精品三区视频，鮑曼不動桿菌的耐藥性呈總體上升趨勢狼狠亚洲综合狼狼久久，耐藥株迅速增加，并出現(xiàn)了多重耐藥株国外黄片免费视频。

The lag growth phase, as well as the stationary phase was delayed with the increase of salinity.

隨著鹽度的增加国产丶欧美丶日本不卡视频，三株菌株生長的延遲期增加，到達(dá)生長穩(wěn)定期的時(shí)間也相應(yīng)增加中国黄色中文字幕。