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單細(xì)胞的受精卵通過卵裂性爱在线观看、桑椹胚、囊胚免费的黄色网站看、原腸胚及器官的發(fā)生等妓女网妓女网站，最后形成一個(gè)能獨(dú)立生活的個(gè)體，涉及發(fā)育和基因表達(dá)的許多基本問題欧美操老女人视频。

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

經(jīng)統(tǒng)計(jì)分析體內(nèi)桑椹胚国产AV高清国片精品、早期囊胚、囊胚的每一胚胎平均細(xì)胞數(shù)與體外桑椹胚欧美视屏在线视频、早期囊胚精品国产一区二区三区91在线、囊胚的每一胚胎平均細(xì)胞數(shù)有顯著差異；擴(kuò)張囊胚的體內(nèi)国产欧美日韩区一区二，體外每一胚胎平均細(xì)胞數(shù)沒有顯著差異成年无码动漫AV片在线观看不卡。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

結(jié)果：①0.1ng/ml、1ng/ml和10ng/ml濃度的LIF對(duì)小鼠早期胚胎的發(fā)育和胚泡的形成有促進(jìn)作用午夜性一级视频，0.01ng/ml和100ng/ml濃度的LIF對(duì)胚胎的發(fā)育無明顯影響国产孕妇一级A片免费观看；②LIF對(duì)小鼠早期胚胎發(fā)育的影響主要發(fā)生于桑椹胚和胚泡期，對(duì)1～8細(xì)胞期胚胎無明顯影響99久久免费精品视香蕉蕉。

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我們測(cè)量的結(jié)果是體內(nèi)2-細(xì)胞早期久久国产热热、2-細(xì)胞晚期、4-細(xì)胞期免费黄网址、桑椹胚国内偷拍专区、囊胚胚胎的EB熒光密度分別是52.92，50.77欧美天天堂在线a一欧，48.84在线观看国产理论播放网站免费视频，39.96和30.36；體外2-細(xì)胞早期aa片黄色视频、2-細(xì)胞晚期亚洲特级黄a、4-細(xì)胞期、桑椹胚久久久久精品高级产三级、囊胚胚胎的EB熒光密度分別是74.85性XXXX18禁免费观看视频，82.62扒开双腿疯狂进出爽爽爽，82.98，81.28和54.09免费在线观看欧美。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球?yàn)楣w克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球?yàn)楣w国产一级免费无病毒黄片、昆明白小鼠MⅡ期卵母細(xì)胞為受體，研究了不同蔗糖濃度對(duì)核移植操作時(shí)間及重構(gòu)胚體外發(fā)育的影響一级欧洲性爱视频。

Here were expression of TGFαand EGFR mRNA in unmatured oocyte, matured oocyte, embryo and the expression of TGFαand EGFR in matured oocytes were comparatively more abundant than that in unmatured oocytes.

GFαmRNA的量在8細(xì)胞期下降到最低水平来个毛片网站，并隨著胚胎基因組的激活胚胎開始轉(zhuǎn)錄TGFα，使TGFαmRNA表達(dá)總量保持在一定水平日本三级片久久，持續(xù)到桑椹胚期6080yy午夜三级中文在线；EGFR mRNA的量在胚胎發(fā)育到桑椹胚期下降到最低，而囊胚期胚胎中的整體表達(dá)量又上升男人深夜天堂网站男人玩游戏女子想要。

With the increase of the 1254 dosages, in the further development process of 2-cell embryo, there still occurred the development relayed and degeneration, so that the development rate of morulae and blastocysts decreascd significantly.

在受精卵的進(jìn)一步發(fā)育中欧美日韩国产一区，隨著公鼠染毒劑量的增加，發(fā)育到桑椹胚和囊胚的比例顯著降低精品亚洲黄色视频在线观看，發(fā)育延遲胚及發(fā)育中退化變性胚比率顯著升高免费观看国产裸体美女网站。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本試驗(yàn)以昆明小鼠1-細(xì)胞期胚胎為實(shí)驗(yàn)對(duì)象，以CZB培養(yǎng)液為基礎(chǔ)液在不同發(fā)育階段添加外源性ROSp怕怕免费网站，培養(yǎng)至桑椹胚99久久精品无码专区、早期囊胚、囊胚91干屄、擴(kuò)張囊胚階段亚州黄色一级无码，分別觀察胚胎的胚細(xì)胞數(shù)、分裂指數(shù)的變化xxxxx在线性爱视频。

In the experiment, the FSH+LH combinations were adopted to superovulate KM mice to ascertain the proper phase to gain morulae and blastocysts, three ways were adopted to split the morulae and blastocysts, demi-embryos were cultured in D-PBS, mTCM199, CZB, mM16 added with FCS or MS of different concentration, and transplantation of the blastocysts derived from demi-embryos was performed.

本試驗(yàn)以昆明白小鼠為實(shí)驗(yàn)動(dòng)物国产视频精神一区，試采用FSH和LH進(jìn)行超排處理，確定了該處理程序下獲取桑椹胚91插插插、囊胚的恰當(dāng)時(shí)間xxxx性欧美，比較了三種方法對(duì)桑椹胚和囊胚分割的效果，采用了添加不同濃度FCS欧美的视频、MS的4種培養(yǎng)基對(duì)分割胚進(jìn)行體外培養(yǎng)国产免费性交a片，并進(jìn)行了半胚移植一a级黄片免费看。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個(gè)部分欧美日韩草草影院在线观看，第一部分對(duì)影響豬卵母細(xì)胞體外成熟和孤雌激活后胚胎分裂的幾個(gè)重要條件進(jìn)行了比較研究，確立了一種較好的培養(yǎng)方法：與顆粒細(xì)胞共培養(yǎng)50熟妇，找到了一種適合豬卵母細(xì)胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸毛片AA级高清免费视频，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%，國(guó)外報(bào)道的最高成熟率為(85.7±4.1)%男人的天堂视频；第二部分對(duì)豬卵母細(xì)胞孤雌激活的化學(xué)方法進(jìn)行了研究强奸乱伦AV片，確立了化學(xué)激活豬卵母細(xì)胞的兩種最佳方法：1將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞用20μmol/Lionomycin作用30min永久女性毛片免费看，再將卵母細(xì)胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中，卵裂率和桑囊胚發(fā)育率達(dá)到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞在200μM/L的Thimerosal中處理20min中文字幕一级限制片，再與8mM的DTT共孵育30min2019人妻巨乳超碰，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%；第三部分對(duì)孤雌激活胚胎的培養(yǎng)條件進(jìn)行了研究亚洲欧洲综合无码一区二区，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡(jiǎn)單培養(yǎng)基中添加顆粒細(xì)胞進(jìn)行前3天的培養(yǎng)理论午夜，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細(xì)胞進(jìn)行后期的培養(yǎng)，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%国产精品青草视频免费播放；第四部分研究了IGF-I