Objective To investigate the effect of different splitting solutions and decompaction treatment on the bisection of mouse embryos at morulae and blastocysts stage and their subsequent transfer after cultured.

目的 研究不同分割液和分割前胚胎去致密化與否對昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影響久久久www黄页免费网站。

The spherical embryonic mass of blastomeres formed before the blastula and resulting from cleavage of the fertilized ovum.

桑椹胚形成于囊胚之前且處于胚胎期內(nèi)的球形裂球群，由受精卵細胞的分裂而成

The survival rates of the early blastocyst and morula treated with 2.5M glycerol were 98.2% and 73.7% respectively.

小鼠桑椹胚和早期囊胚用2.5M甘油處理存活率分別為73.7％和98.2％舔穴在线。

At the same time, a fertilized ovum to the uterine cavity gradually moving ,3-4 days after the arrival of intrauterine growth when cells become more than an entity, shaped like mulberry, so called morula.

與此同時91精品免费大秀，受精卵逐漸向?qū)m腔方向移動，3—4天后到達宮腔時已發(fā)育成為一個具有多個細胞的實體www.tsztjs.com，形狀象桑椹黄免费片网站入口处，所以稱為桑椹胚。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

結(jié)果：與無上皮細胞相比最新色色网址，共培養(yǎng)明顯促進了小鼠桑椹胚率（71.3%和61.2%国产v碰，p.05），囊胚率（50.0%和39.7%亚洲免费送视频，p.05）色94色欧美一区，囊胚孵化率（34.8%和24.3%，p.05）和胚胎總細胞數(shù)（44.1和35.6你懂网站在线，p.05）99九九热爱视频精品免费。結(jié)論：子宮內(nèi)膜上皮細胞能夠促進小鼠胚胎的體外發(fā)育，對早期胚胎的體外發(fā)育環(huán)境具有優(yōu)化作用无码人与动欧交视频A片。

After lOmin delay only 81.2% pronucleus developed to blastula, significantly lower than control (93.2%).

滯留20min不引起原核胚久久wwwcom、2-cell胚胎和桑椹胚以及囊胚的死亡。

The aim of the present study was to isolate and culture ESCs and EGCs from goat embryos and fetuses of different development stage, and to compare some factors influencing the efficiency of establishing those stem cell lines by adopting the methods of mouse and human. The results obtained were as follows: 1. A total of 46 Guanzhong dairy goats were superovulated with a result of total 307 embryos including 39 embryos of 4-cell to 16-cell, 128 morulae, 89 blastocysts and 51 hatched blastocysts. In the study, we obtain 6.67 embryos from per goat.

本研究選用關(guān)中奶山羊胚胎和胎兒為試驗對象日韩片婬乱一级毛片视频，參照小鼠和人ESCs和EGCs建系的方法日韩在线观看网红自拍视频网站免费，系統(tǒng)比較了不同培養(yǎng)條件和傳代方法對分離培養(yǎng)山羊ESCs和EGCs的影響，主要獲得以下結(jié)論： 1本試驗共超排處理了46只/次關(guān)中奶山羊国产盗摄在线一区二区三区，共獲得胚胎307枚91se国产在线视频，其中4~16細胞胚胎39枚，桑椹胚128枚国产欧美亚洲91精品，囊胚89枚农村少妇A级毛片免费看，孵化胚51枚，平均每只山羊獲得6.67枚胚胎亚洲精品国产2014AV天堂网手机版。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h激情亚洲AV无码日韩色，不影響卵母細胞的成熟和孤雌激活能力国产精品香蕉AV，并且CHX抑制后再成熟的卵母細胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對照組卵母細胞相似。8久久精品久久久久久久久、體外受精后精品久久99国产精品，CHX抑制后再成熟卵母細胞的多精受精現(xiàn)象顯著增加，發(fā)育到桑椹胚/囊胚的比例顯著低于對照組国产区在线。9久久久久久黃色網站、CHX抑制過程中皮質(zhì)顆粒仍能發(fā)生遷移，但是CHX抑制會對皮質(zhì)顆粒的遷移造成不可恢復的損傷国内亚洲精品无码久久，使再成熟的卵母細胞內(nèi)皮質(zhì)顆粒不能完全遷移国产免费播放网站。10、CHX抑制對山羊卵母細胞α-微管和微絲都沒有影響国产精品亚洲一区二区动漫，無論是抑制后處于生發(fā)泡期的卵母細胞免费A片大片aⅴ免费，還是抑制后再成熟的卵母細胞，微管和微絲的分布都與對照卵母細胞相似精久视频69视频在线观看。

Methods C 57BL/6J strain female mice mated with KM male mice, after seeing plug 3.5 da ys the compacted morulae and blastocysts were collected and bisected microsurgic ally by using special knife, obtained half embryos were cultured and observed.

C57BL/6J雌性小鼠與KM雄性小鼠交配国产精品三级黄色岛国AⅤ免费看，見栓3.5d采集小鼠晚期桑椹胚和早期囊胚，應用顯微手術(shù)刀直接分割法進行胚胎二等份分割成年人在线免费观看热视频，所得半胚體外培養(yǎng)日本小视频网站，觀察其發(fā)育情況。

The cleavage rate of 2-cell stage was 50%, but few embryos developed into morulas and blastocysts (2%). More than 130 embryos at 2-4-cell stage were transferred into oviduct of 13 synchronized foster rabbits and 2 mice. No fetus or implantation sites were observed in both recipients.

其2—細胞胚胎的分裂率可達50％一区二区国产黄片，但發(fā)育至桑椹胚和囊胚階段的胚胎數(shù)目大大減少久久99视频，只有2％的囊胚發(fā)育率。13只受體兔共移植重構(gòu)胚胎121枚插插插射射射，結(jié)果未發(fā)現(xiàn)產(chǎn)仔或死胎夜色樱花网站，僅看到有的兔子宮有於血狀。