Furthermore, when glucose was introduced into the CZB medium on the third day of the culture, the development rates of over morula and blastocyst(66.7%,28.1%) were improved significantly.

進一步，于培養(yǎng)的第3 d在CZB培養(yǎng)液中加入葡萄糖，其桑椹胚以上及囊胚發(fā)育率明顯提高（66.7%、28.1%）。

The survival rates of early blastocyst treated with trehalose and sucrose, were 79.1%, and.77.1%, respectively; and the survival rates of morula were 46.3%, and 51.6%, respectively.

用海藻糖和蔗糖處理的小鼠早期囊胚存活率分別為79.06％和77.07％又长又大又硬黄色；桑椹胚分別為46.31％和51.63％。

The survival rates of mice early blastocyst and morula treated with 0.5M trehalose were 61.8% and 95.6% respectively; and treated with 0.25M trehalose were 35.5% and 67.2% respectively.

小鼠桑椹胚和早期囊胚用0.5M海藻糖處理后存活率分別為61.84％和95.57％；用0.25M海藻糖處理后存活率分別為35.50％和67.22％亚洲一区精品黄。

Morula in the uterine cavity to continue cell division to form the blastocyst, about 6-8 days after fertilization into the endometrium, a process known as implantation or cultivation.

桑椹胚在子宮腔內(nèi)繼續(xù)細胞分裂形成胚泡一级做a爰片久久毛片A片美国，大約在受精后6—8天進入子宮內(nèi)膜，這個過程叫做著床或種植欧美性爱在线播放久草。

After a continuous culture for 24 h, 77.1%(81/105)of the reconstituted embryos developed to 2-cell stage; The developmental rates of 4-celland morula were 45.7%(48/105) and 17.1%(18/105), respectively.

體外繼續(xù)培養(yǎng)24h后国产片黄片A片，77.1％(81／105)的重構(gòu)胚發(fā)育到2-細胞；4-細胞和桑椹胚的發(fā)育率分別達到45.7％(48／105)和17.1％(18／105)亚洲一级av无码毛片精品。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB一级欧美性爱网站，可明顯提高雙原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚發(fā)育率（40.7％vs 26.8％黄色AⅤ，P＜0.05）天天黄色视频入口，但對其激活分裂率和總原核形成率無明顯影響（P＞0.05）。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

總之18岁网站。，我們的研究表明插逼网址，DET玻璃液冷凍方法是簡單、經(jīng)濟而又高效的久久国产综合精品久久国产，冷凍小鼠各階段胚胎都能獲得較高的存活率国产精品手机在线，其中受精卵的EDT30二步法1分鐘平衡組(66％)、桑椹胚的EDT30二步法1分鐘平衡組(94％)和EDT40二步法0.5分鐘平衡組(94％)xx69欧美、囊胚的EDT30二步法1分鐘平衡組(91％)和EDT40一步法1分鐘平衡組(91％)冷凍效果最好。

The fusion of donor-recipient pairs was induced with different parameters for electrofusion with variation in electric field intensity, pulse duratio...

在不同電融合條件下誘導(dǎo)兩者間的融合成年人视频www，并對重組胚激活以及隨后發(fā)育的早期階段包括2細胞操来操去网站、4~8細胞和桑椹胚進行觀察和計數(shù)。

The expression of subunits α5,β1,β3 was detected in normal morulas and increased in blastocysts. The expression of subunit α1 was not detected in morulas or blastocysts, but detected only in outgrowing trophocytes. 3. Expression of integrin β3 in irradiated group embryos was weaker than that of normal group. There was no expression of integrin α1 both in morulas and blastocysts in irradiated group and no difference in the expression of subunits α5 and β1 between these two groups.

結(jié)果：1欧美孕妇与黑人孕交4p、超聲波照射組胚胎貼附率久久精品视频里只有精品、滋養(yǎng)細胞外延生長率均顯著低于正常胚胎。2免费黄色网站有、正常胚胎桑椹胚期即有α5XXXX性欧美极品V、β1、β3整合素表達日本在线中文字幕播放，而無α1表達超级超碰；囊胚期α5、β1欧美人与动性行为高清视频、β3表達增強玖玖玖玖玖无码，仍無α1表達；α1只出現(xiàn)在外延生長的滋養(yǎng)細胞午夜中文不卡理伦片。3一区黄色片A级、超聲波照射組胚胎β3整合素的表達明顯弱于正常組，且未見α1表達；而α5中文字幕视频网、β1的表達兩組間無差異久草1。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦顯微鏡檢測細胞色素C結(jié)果顯示：在桑椹胚期和囊胚期，隨著BPA處理濃度的升高操美女黄色网站免费观看，釋放細胞色素C的胚胎比率逐漸增加欧美高颜值A片在线播放，說明細胞色素C參與了BPA誘導(dǎo)胚胎細胞凋亡的過程，這一過程是通過線粒體通路來調(diào)節(jié)的meiav色在线。