A independent life individuality is finally formated from singlecell ferti1ized egg throngh egg cleavage、morula stage、blastocoele stage、protogaster stage and orange etc, and concerned in major basal questions of development and gene expression.

單細胞的受精卵通過卵裂、桑椹胚亚洲一区二区免费视频网站、囊胚、原腸胚及器官的發(fā)生等美女被操网站免费不卡，最后形成一個能獨立生活的個體欧美黄色免费在线观看，涉及發(fā)育和基因表達的許多基本問題。

The spherical embryonic mass of blastomeres formed before the blastula and resulting from cleavage of the fertilized ovum.

桑椹胚形成于囊胚之前且處于胚胎期內的球形裂球群av一本久道久久波多，由受精卵細胞的分裂而成

Morula in the uterine cavity to continue cell division to form the blastocyst, about 6-8 days after fertilization into the endometrium, a process known as implantation or cultivation.

桑椹胚在子宮腔內繼續(xù)細胞分裂形成胚泡综合网图，大約在受精后6—8天進入子宮內膜，這個過程叫做著床或種植国产黄色视频观看。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

結果：①0.1ng/mlA级毛片无码免费、1ng/ml和10ng/ml濃度的LIF對小鼠早期胚胎的發(fā)育和胚泡的形成有促進作用，0.01ng/ml和100ng/ml濃度的LIF對胚胎的發(fā)育無明顯影響国内精品久久久亚洲；②LIF對小鼠早期胚胎發(fā)育的影響主要發(fā)生于桑椹胚和胚泡期亚洲图片视频小说，對1～8細胞期胚胎無明顯影響。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB丁香五月激情黄色操一操，可明顯提高雙原核的形成率（38.9％vs 15.1％色www，P＜0.05）和桑椹胚/囊胚發(fā)育率（40.7％vs 26.8％，P＜0.05）日本黄色视频mmm，但對其激活分裂率和總原核形成率無明顯影響（P＞0.05）亚洲另类专区欧美。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個部分，第一部分對影響豬卵母細胞體外成熟和孤雌激活后胚胎分裂的幾個重要條件進行了比較研究欧美乱人伦中文视频在线，確立了一種較好的培養(yǎng)方法：與顆粒細胞共培養(yǎng)在线超碰人人摸，找到了一種適合豬卵母細胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%国产欧美很黄，國外報道的最高成熟率為(85.7±4.1)%A片集合；第二部分對豬卵母細胞孤雌激活的化學方法進行了研究，確立了化學激活豬卵母細胞的兩種最佳方法：1將成熟的去卵丘顆粒細胞的MII期卵母細胞用20μmol/Lionomycin作用30min黄色A片洗澡，再將卵母細胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中，卵裂率和桑囊胚發(fā)育率達到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細胞的MII期卵母細胞在200μM/L的Thimerosal中處理20min久久九九久91精品国产综合，再與8mM的DTT共孵育30min黄色视频在做在线，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%；第三部分對孤雌激活胚胎的培養(yǎng)條件進行了研究免费欧美操屄，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細胞進行前3天的培養(yǎng)亚洲另类日韩，然后轉入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細胞進行后期的培養(yǎng)，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%嗯啊快点视频在线观看；第四部分研究了IGF-I