Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合人人看黄视频、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程日本强伦姧人妻一区二区，在細(xì)胞質(zhì)融合的時(shí)候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣成人影院蜜桃传媒在线播放，呈現(xiàn)高度甲基化狀態(tài)瑟瑟在线人体；在細(xì)胞核開始融合的時(shí)候，甲基化水平急速下降全色色网2021，在細(xì)胞核完全融合的時(shí)候甲基化水平達(dá)到最低點(diǎn)免费三级电影网；隨著胚胎繼續(xù)分裂，胚胎甲基化水平逐漸增加av国岛无码专区人妻系列，在桑葚胚期甲基化水平最高一级a免一级a做视频观看；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團(tuán)同滋養(yǎng)層細(xì)胞甲基化熒光信號(hào)沒有差別，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團(tuán)細(xì)胞甲基化熒光強(qiáng)度高于滋養(yǎng)層細(xì)胞甲基化熒光強(qiáng)度不同黄片免费视频精品免费视频。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

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Rabbit(Oryctolagus cuniculus domestica); mRNA differential display ; Morula stage embryo ; Zygotic gene

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Germ potential is established after conception in a totipotent zygote and retained subsequently during development in blastomers of morula , cells form the inner cell mass of blastocyst , epiblast and population of primordial germ cells .

種子潛能的確定是全能的受精卵形成之后黄色视频免费看欧美中文字幕的，并且隨后在桑葚胚，胚泡內(nèi)層細(xì)胞外国二级黄色二级黄色视频外国，外胚層和原生殖細(xì)胞的發(fā)育過程中保留日日射天天操。

Aggregation of ES Cells with Mouse Morula state Embryos .

聚集的ES細(xì)胞與小鼠桑葚胚國(guó)家的胚胎。

The morula slowly moves down the Fallopian tubes toward the uterus, where it arrives after about three days.

桑葚胚慢慢地沿輸卵管向下国产大学生性爱视频、朝子宮移動(dòng)十八禁止免费网站，到達(dá)那里約需3天。

The zygote, by cell division, grows into a morula an then into a blastocyst.

通過細(xì)胞分裂男女无遮挡毛片视频免费播放，受精卵發(fā)育成桑葚胚亚洲人人看a，然后發(fā)育成囊胚。

It transforms the zygote into a cluster of cells called morula which, seen through a microscope, resembles a mulberry.

卵裂把受精卵轉(zhuǎn)換成稱為桑葚胚的細(xì)胞團(tuán)好色干亚洲，它在顯微鏡下看起來象桑葚果日本三级片免费在线。

With the prolonging of treatment with 6-DMAP,the developmental ability of parthenogenetic embryos decreased.

隨著6-DMAP處理時(shí)間加長(zhǎng)，孤雌胚的發(fā)育能力降低www.a免费黄片。6-DMAP處理1小時(shí)的桑葚胚／囊胚的發(fā)育率(72％)遠(yuǎn)遠(yuǎn)高于6-DMAP處理6小時(shí)的桑葚胚／囊胚的發(fā)育率(28％)亚洲精品福利网。

There is similarity with the growth of the cancer cell in the growth characteristic of the mulberries embryo in embryo's development course.

胚胎發(fā)育過程中桑葚胚的生長(zhǎng)特征與癌細(xì)胞的生長(zhǎng)有相似之處欧美肛交一级黄色片。