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Methods:32 patients of TCPC were reviewed: the average age was 10 years,ranged from 5 to 24 years and the average weight was 28 kilogramme,ranged from 13 to 55 kilogramme.18 patients underwent lateral tunnel and 1 patient underwent extracardiac conduit TCPC with hypothermic cardiopulmonary bypass and heart arrest,and 10 patients were treated by extracardiac conduit technique with normothermic CPB and without heart arrest and 2 patients without CPB,and 1 patients underwent two-step operation.

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Coning and quartering method: put the sample onto the steel plate in the form of a cone; each scoop shall be inserted from the top of conical to make it spread along the top evenly, and pay attention not to misplace the center of cone; do it at least 3 times and mixed thoroughly; then flatten the top and use the cross plate from top to bottom to divide into 4 equal segments, randomly take out 2 oppositesegments and do it repeatedly for several times and finally divided into not less than the minimum retained quantity.

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