Methylation is negative correlation with genetic transcription , and the mechanism is probably as follows : directly interfere the combination of specific transcripton and recognition site of each promoter ; mediated transcription inhibition by change the construction of chromatin; induced gene silencing by combination of methylation site and transcription inhibition. P16 gene is also called multiple tumor supresser gene , which is the best easily to methylate in the anti-oncogenes. P16 gene locates in 9p21 of mankind-chromatosome , and it's total length is 8.5kb .

甲基化與基因轉(zhuǎn)錄呈負相關(guān)欧美黑白性爱视频，其機制可能是[3]：直接干擾特異的轉(zhuǎn)錄子和各自啟動子識別位點的結(jié)合；通過改變?nèi)旧|(zhì)結(jié)構(gòu)欧美性爱自拍小视频，介導轉(zhuǎn)錄抑制黄视频官方网站；甲基化DNA位點直接與轉(zhuǎn)錄抑制子結(jié)合，誘導基因沉默人妻视频在线精品网站免费。p16基因又稱為多腫瘤抑制基因( multiple tumor suppressor 1 www.大色网.com，MTS1) [4] ，是肺癌中最易發(fā)生甲基化的抑癌基因，該基因位于人類染色體9p21上视频高清国产在线观看网站下载安装，全長8.5kb 夜夜大片网，由兩個內(nèi)含子和三個外顯子組成。

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

應(yīng)用甲基轉(zhuǎn)移酶抑制劑5-氮雜胞苷(5-azacytidine欧美AA视频网站， 5-azaC)可以使甲基化的啟動子去甲基化欧美国精品亚洲精品日韩专区，啟動基因重新轉(zhuǎn)錄；高濃度同型半胱氨酸(homocystineA三级片免费网站， Hcy)可以介導基因甲基化而抑制其表達日韩草逼精品毛片。

Although the biological significance of DNA methylation has been recognized to some extent, the mechanisms of DNA methylation regulation and of the establishment of tissue-specific DNA methylation pattern in somatic cells are still unclear, since the knowledge of the enzymes responsible for de novo DNA methylation and demethylation of DNA is very limited and up to now only two functional DNA methyltransferases in mammals have been cloned.

目前，雖然對DNA甲基化的作用已有一定認識在线A片毛视频，但DNA甲基化的調(diào)節(jié)以及發(fā)育過程中組織特異的DNA甲基化譜的建立機制還不清楚国产91免费在线。問題主要在于對催化重新甲基化和去甲基化反應(yīng)的酶的了解很少。至今僅克隆出兩種有功能的哺乳動物DNA甲基轉(zhuǎn)移酶九九r热網站。

The complexity of the methylarginine mark is enhanced by the ability of this residue to be methylated in three different ways on the guanidino group: monomethylated, symmetrically dimethylated and asymmetrically dimethylated, each of which has potentially different functional consequences.

根據(jù)將S-腺苷甲硫氨酸(S-adenosylmethioninewww.99riavcom，AdoMet)上的甲基轉(zhuǎn)移到精氨酸的胍基氮上產(chǎn)生甲基化的不同方式，最終可以分為三類：單甲基化女人高潮一级AA片视频、對稱性雙甲基化和非對稱性雙甲基化老嫖客精品视频在线播放免费。

The remainder 12 patients were fourd Id4 unmethylated, 1 case out of whom relapsed within the next 3-month of follow-up (8.33%), while 10 out of 20 patients (50%) with 1d4 methylation relapsed within the same follow-up period.

結(jié)果發(fā)現(xiàn)，32例完全緩解的ALL患者中20例(62.5%)DNA抑制因子4呈甲基化色色色色鬼。12例DNA抑制因子4呈非甲基化的患者中只有1例3個月內(nèi)復(fù)發(fā)综合区图片区视频区亚小说区，復(fù)發(fā)率8.33%，20例DNA抑制因子4呈甲基化狀態(tài)的患者中10例3個月內(nèi)復(fù)發(fā)日韩精品无码露脸视频迅雷下载，復(fù)發(fā)率50%在线国产主播专区第一页。

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

設(shè)計1組特殊熒光標記探針以構(gòu)建1種檢測p16基因啟動子區(qū)3個CpG位點甲基化改變的芯片，特殊熒光標記探針包括1對非甲基化探針和甲基化探針性ⅩXXXXHD欧美精品，檢測相鄰的3個CpG位點甲基化的程度久久久久久免费精品电影网。

Methods genomic dna was extracted from different stages of malignant transformation and tumorigenicity in inoculated athymic mouse 16hbe cells induced by cadmium chloride and methylating status of genome p16 gene promoter was observed with methylation-specific pcr and then compared with the controls.

從各組cdcl2惡性轉(zhuǎn)化不同階段及接種裸鼠成瘤的16hbe細胞中提取全基因組dna，采取甲基化特異性pcr法(methylation-specific pcr欧美又黄又大又粗大片，msp)檢測該基因組p16基因啟動子區(qū)的甲基化狀況国产又粗又猛又爽的野外视频，與非轉(zhuǎn)化的16hbe對照細胞進行比較，并用去甲基化因子5-azac (5-aza-2'deoxycytidine)處理有異常甲基化的細胞5要A片欧美无线看。

E-cadherin methylation may play an important role in the carcinogenesis of colon carcinoma cells and can re-express after the treatment with 5-Aza-CdR.

E-cadherin基因啟動子區(qū)異常甲基化是結(jié)腸癌發(fā)生亚洲欧洲美洲无码精品Va水蜜桃、發(fā)展的重要機制之一，5-Aza-CdR能使甲基化的E-cadherin去甲基化修飾并重新表達欧美视频一区二二区。

Theoretically, re-expression of tumor suppressor genes by demethylating agents may contribute to inhibiting the development of tumor. It has been suggested that DNA hypermethylation results in resistance to chemotherapy and consequently reduces the efficacy of chemotherapeutic treatment.

由于高度甲基化而暫停表達的抑癌基因欧美一级淫片在线观看，如果能應(yīng)用去甲基化制劑逆轉(zhuǎn)甲基化的異常狀態(tài)，使之重獲表達精品视频黄色，理論上即可發(fā)揮其抑制腫瘤細胞生長的功能亚洲一区二区三区性爱视频。

Krogan N J, Kim M, Tong A, et al Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase ||. Mol Cell Biol, 2003, 12(2):476-487[12] J Biol Chem.

表達基因轉(zhuǎn)錄起始下游的局限性H3-K9的三甲基化不在啟動子高甲基化的區(qū)域视频偷拍，H3-K9的三甲基化耦合于RNA聚和酶II在沉默基因中具有一致性。