Results 25 protuberances from outside organ (spleen, n=19; aorta, n=4; left hepatic lobe, n=1 and cyst of pancreatic tail, n=1) and 92 SMTs were detected by EUS including 63 myogenic ones (esophageal, n=34; gastric, n=29), 9 etopic pancreases in stomach, 8 esophgeal cysts, 3 varices in gastric fundus, 3 gastric lymphomas, 2 gastric lipomas, 2 Menetrier ' s disorders, 1 esophageal lipoma and varix each.

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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

應(yīng)用雜交瘤技術(shù)制備ZNRD1的首個(gè)單克隆抗體116美女极品A级毛片；2）利用RT-PCR、Western blot和免疫組化檢測(cè)ZNRD1在胃癌組織肉翻闷湿OL丝袜在线播放、胃炎組織9999国产精品视频、正常胃上皮組織、胃癌細(xì)胞和正常胃組織上皮細(xì)胞中的表達(dá)在线无码不卡加勒比HEYZO；3）構(gòu)建ZNRD1的小干擾RNA載體jizzjizz国产精品，并測(cè)序鑒定；4）利用脂質(zhì)體將ZNRD1的真核表達(dá)載體及其空載體轉(zhuǎn)染胃癌細(xì)胞（AGS欧美性爱v、SGC790191中国老熟女的大屁股、MKN28）和小鼠成纖維細(xì)胞（NIH3T3），G418篩選后進(jìn)行鑒定аⅴ天堂中文在线；5）利用脂質(zhì)體將ZNRD1的小干擾RNA載體及其空載體轉(zhuǎn)染藥敏胃癌細(xì)胞（SGC7901）激情图库、正常胃組織上皮細(xì)胞（GES-1）、對(duì)長(zhǎng)春新堿耐藥的胃癌細(xì)胞（SGC7901/VCR）AⅤ激情网、藥敏白血病細(xì)胞（HL-60）天天色天天操天天摸天天抽、對(duì)長(zhǎng)春新堿耐藥的白血病細(xì)胞（HL-60/VCR）黄片一级免费观看，G418篩選后進(jìn)行鑒定超碰在线8888；6）利用MTT實(shí)驗(yàn)檢測(cè)ZNRD1高/低表達(dá)對(duì)細(xì)胞（AGS、SGC7901日韩综艺精品视频在线观看、MKN28日韩交换精品一区二区、NIH3T3、GES-1）生長(zhǎng)的影響caopeng视频在线；7）通過軟瓊脂克隆形成實(shí)驗(yàn)檢測(cè)上調(diào)ZNRD1對(duì)AGS国产免费观看黄页影视网站大全、MKN28細(xì)胞克隆形成能力的影響；8）通過裸鼠成瘤實(shí)驗(yàn)檢測(cè)上調(diào)ZNRD1對(duì)AGS亚洲Av片久久综合网、MKN28細(xì)胞體內(nèi)成瘤性的影響国产精品久久久久精品三级涩；9）通過流式細(xì)胞儀分析ZNRD1高/低表達(dá)對(duì)細(xì)胞（AGS、MKN28成人一级黄色视频网站、NIH3T3国产又黑又粗又爽的免费视频、GES-1）的細(xì)胞周期的影響；10）通過流式細(xì)胞儀分析上調(diào)ZNRD1對(duì)細(xì)胞（AGS欧美视频性一区、MKN28AAA黄色网址、NIH3T3）的凋亡的影響欧美激情四射xXXX888；11）通過MTT實(shí)驗(yàn)檢測(cè)ZNRD1高/低表達(dá)對(duì)細(xì)胞（SGC7901、SGC7901/VCR婷婷五月丁香社、HL-60日日碰人人操、HL-60/VCR）體外藥物敏感性的影響；12）通過腎包膜下移植法檢測(cè)ZNRD1高/低表達(dá)對(duì)細(xì)胞（SGC7901免费网站看a级来、SGC7901/VCR）體內(nèi)藥物敏感性的影響国产一级黄片视频久久baoyu；13）通過流式細(xì)胞儀分析ZNRD1高/低表達(dá)對(duì)細(xì)胞（SGC7901、SGC7901/VCR一级一级α片免费看在钱、HL-60人人看人人干人人看、HL-60/VCR）內(nèi)阿霉素蓄積和泵出的影響；14）通過透射電鏡檢測(cè)上調(diào)ZNRD1對(duì)SGC7901細(xì)胞凋亡敏感性的影響色综合视频在线导航；15）通過流式細(xì)胞儀和DNA梯度試驗(yàn)檢測(cè)ZNRD1高/低表達(dá)對(duì)細(xì)胞（SGC7901亚洲一级黄色录像、SGC7901/VCR、HL-60）凋亡敏感性的影響特黄片毛片；16）通過基因芯片檢測(cè)ZNRD1高/低表達(dá)對(duì)胃癌細(xì)胞內(nèi)基因表達(dá)譜的影響操操日小说；17）利用RT-PCR、Western blot對(duì)基因芯片的結(jié)果進(jìn)行鑒定年轻人看的黄色视频人妻无码；18）利用報(bào)告基因?qū)嶒?yàn)檢測(cè)ZNRD1對(duì)cyclin D1的啟動(dòng)子活性的調(diào)節(jié)作用美女被干黄色网站；19）利用報(bào)告基因?qū)嶒?yàn)檢測(cè)ZNRD1高/低表達(dá)對(duì)MDR1的啟動(dòng)子活性的調(diào)節(jié)作用；20）利用激酶試驗(yàn)檢測(cè)ZNRD1對(duì)cyclin E-CDK2 激酶活力的影響www欧美com；21）利用反義核酸技術(shù)抑制p21的表達(dá)www.wuma04.com；通過流式細(xì)胞儀檢測(cè)抑制p21對(duì)ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響；22）利用反義核酸技術(shù)抑制p27的表達(dá)尤物视频139；通過流式細(xì)胞儀檢測(cè)抑制p27對(duì)ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響玖玖色影院；23）利用脂質(zhì)體轉(zhuǎn)染法上調(diào)Skp2的表達(dá)；通過流式細(xì)胞儀檢測(cè)上調(diào)Skp2對(duì)ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響最新av网址在线；24）利用Western blot檢測(cè)ZNRD1對(duì)p27和Skp2的蛋白穩(wěn)定性的影響欧美日韩视频在线免费观；25）利用微血管密度實(shí)驗(yàn)檢測(cè)ZNRD1對(duì)AGS、MKN28細(xì)胞裸鼠移植瘤微血管形成的影響欧洲激情无码；26）利用ELISA檢測(cè)ZNRD1對(duì)AGS色诱久久av无码电影、MKN28細(xì)胞培養(yǎng)上清和移植瘤勻漿中VEGF165含量的影響；27）利用脂質(zhì)體轉(zhuǎn)染法大陆老熟女乱人伦视频、MTT實(shí)驗(yàn)污小蝌蚪视频在线观看、腎包膜下移植法、流式細(xì)胞儀和DNA梯度試驗(yàn)檢測(cè)新耐藥相關(guān)分子DARPP-32對(duì)細(xì)胞（SGC7901A片二区图、SGC7901/VCR去看99久久黄色一级片、對(duì)阿霉素耐藥的胃癌細(xì)胞SGC7901/ADR）多藥耐藥表型的影響；利用脂質(zhì)體轉(zhuǎn)染法和MTT實(shí)驗(yàn)檢測(cè)下調(diào)ZNRD1對(duì)DARPP-32介導(dǎo)的胃癌多藥耐藥的調(diào)控作用久久器只。

Straw ; corn oil ; rumen degradability ; digestibility

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The results showed that the variance in stem shearing force of Alfalfa and rye grass are due to a combination of factors, including changes in chemical composition, morphological characteristics. DM and NDF degradability of alfalfa stem had negative relationships with shearing force. Shearing force could be used as an indicator of forage stem nutritive value.

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This paper studies on the effects of Allium mongolicum regel and oilseeds on rumen fermentation , nutrient passage , digestion and meat quality in sheep.

本論文系統(tǒng)全面地研究了日糧中添加沙蔥和油料籽實(shí)對(duì)瘤胃發(fā)酵欧美at在线、營(yíng)養(yǎng)物質(zhì)流通消化及胴體品質(zhì)的影響在线视频播放社区。

The effects on separation and identification of Allium Mongolia Regel Extractive and its effects on CLA contents in digest tract, fat deposition of carcass and mutton flavor precursors in sheep were studied systematically in the thesis, which implements for synthesizing man-made Allium Mongolia Regel extractive .

本論文系統(tǒng)全面地研究了沙蔥提取物的分離鑒定及其對(duì)綿羊瘤胃發(fā)酵、消化道共軛亞油酸含量特级精品AAAAAAA片、胴體脂肪沉積和羊肉風(fēng)味前體物的影響九九小视频，為人工合成沙蔥提取物提供試驗(yàn)依據(jù)。

The nylon bag technique was used to determine the 48h and 72h rumen degradabilities of DM and NDF of wild rye, wheat straw and rice straw.

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The nylon bag technique was used to determine the 48 h and 72[ KG*9]h rumen degradabilities of DM and NDF of wild rye, wheat straw and rice str aw. The digesitbility of dietary DM, NDF or CP in the whole digestive tract was also determined.

用尼龍袋技術(shù)分別測(cè)定羊草95福利人人干人人摸、麥秸和稻草的干物質(zhì)和中性洗滌纖維48 h和72 h的瘤胃降解率和日糧DM一站式A片，NDF和粗蛋白的全消化道消化率。

Forage with much small particle size could decrease nutrient digestion in the rumen and the small intestine and increase nutrient digestion in the hindgut of sheep.

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However, group Ⅲ was the lowest for solid phase passage rate, and fiber digestibility in rumen and whole digestive tract, but the fiber digestion was enhanced in the hindgut of sheep.

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