Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.

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Methods Hyperlipaemia and AP model were established with Triton WR1339 and Cerulein respectively. AP with hyperlipaemia model was prepared by both Triton WR1339 and cerulein simultaneously. Human albumin was used to treat the AP accompanied with hyperlipaemia. In each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. The level of protein kinase C membrane translocation in pancreatic tissue was detected by Western blot.

分別用Triton WR1339、Cerulein制作大鼠高脂血癥和急性胰腺炎（acute pancreatitis天天透天天狠狠狠的，AP）模型国产精品不卡无码AV在线播放，同時(shí)應(yīng)用兩者制作伴有高脂血癥的AP模型，應(yīng)用白蛋白治療伴有高脂血癥的AP亚州一级特黄片免费视频在线，比較各組胰腺病理?yè)p害評(píng)分亚洲自偷自偷图片在线、腹水量国产公开免费视频、胰腺濕/干比、血清淀粉酶和胰腺組織的凋亡久久婷婷五月综合品精；Western blot檢測(cè)胰腺組織PKC的膜轉(zhuǎn)位免费性爱黄色视频。

Methods Modification I:Transverse incision was chosen on epigastirc,a piece of about 3×5 cm fascil strip with pedical as well as bilateral anterior and posterior sheath of recus and peritoneum was pulled through transverse mesocolon functioning as the supporting stick of traditional loop ostomy.The colon of ostomy was buried subcutaneously,stoma was opened instantly or secondarily depending on the patients′s condition.

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By using red blood cell broken-up solution,proteinase K and RNase,total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species,Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.

本實(shí)驗(yàn)以河南董寨自然保護(hù)區(qū)白冠長(zhǎng)尾雉和環(huán)頸雉已孵化的卵殼為材料日本一区二区在线播放，利用紅細(xì)胞破碎液、蛋白酶K及RNA酶等試劑女人18特级一级毛片免费视频，對(duì)卵殼膜內(nèi)的總DNA進(jìn)行了提取中文字幕日韩电影在线，建立了一種提取高質(zhì)量DNA的新方法。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

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It shows that the average thickness of the surface layer of crystals,the palisade and cone layer and the eggshell membrane are 20.8,220.8 and 62.5μm,respectively,which accounting for 6.8%,72.6% and 20.6% of the total thickness of the eggshell.There are many vesicular holes in palisade layer with an average diameter of(0.32±0.08)μm(n=30).The function of such holes may be of significant to air exchange.The shape of eggshell pore on the surface layer of crystals is round or ellipsis.The fracture surface of the pore is funnel-shaped.

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