To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

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In this thesis, two synthetic routes for the incorporation of sugar moiety into C5(5") or C3(3") of salicylaldehyde part of Salen Mn complex were designed and several corresponding compounds were prepared, as carbohydrate derivatives are widely used in asymmetric catalytic reactions.

鑒于糖類衍生物在不對稱催化反應中的廣泛應用黑硬大长欧美在线视频，設計了兩條分別在Salen Mn配合物水楊醛部分C5(5′)免费黄色xxxx视频、C3(3′)位并入糖基的路線并合成了相應的化合物。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通過對差減文庫篩選这里只有精品99久久精品国产，分離到59個對菌株VIB72的克隆欧美另类激情视频在线观看，并對這些克隆的DNA序列進行了測定。17個基因片斷與其它細菌的已知功能的基因有較高的同源性官产在线免费观看无码，其中包括可溶性溶胞壁質(zhì)轉糖基酶中文字幕国产无码、轉移蛋白MobA和MobC、轉座子IS66超碰人妻97精品、抑制相關蛋白（金屬β-內(nèi)酰胺酶和乙酰轉移酶家族）日本欧美国产激情、毒素蛋白（DT-201和alveicin A免疫蛋白）、與OLD 家族相似的ATP依賴性核酸內(nèi)切酶以及SocE 和GTP結合蛋白HflX三A黄色网站。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分離正常人臍靜脈內(nèi)皮細胞我想看黄色一级高清无码视频，將晚期糖基化終產(chǎn)物修飾的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培養(yǎng)基與HUVEC在體外共同培養(yǎng)免费看的黄色视频，用熒光單克隆抗體染色99riav1com，流式細胞儀定量檢測內(nèi)皮細胞表面細胞間粘附分子-1（ICAM-1）、血管細胞間粘附分子-1（VCAM-1）九九久精品、E-選擇素的表達中文字母强奸乱论。

The position, type and number of sugar moieties incorporated to the antibiotics have great impact on its bioactivity.

本文綜述了糖基轉移酶的種類、功能cao96、特性及其在組合生物合成中的應用與研究前景曰批全过程免费视频下载。

To study the effects of glycosylation on survival of cold-storage human platelets by using rabbit model.

利用兔動物模型研究了糖基化對4℃冷藏保存人血小板體內(nèi)生存的影響。

The expression patterns of expansin and XTH family genes in cucumber fruits and their responses to plant growth substances were studied.

研究了擴張蛋白和木葡聚糖內(nèi)糖基轉移/水解酶家族基因在黃瓜果實發(fā)育中的作用及其對植物生長物質(zhì)的響應欧美日韩精品一二三区。

Abstract］ Objective To evaluate the correlation between soluble receptopr for advanced glycation end products and coronary artery disease, the etent and severity of angiographic CAD in nondiabetic patients.

目的 探討可溶性糖基化終產(chǎn)物受體水平與無糖尿病冠心病的發(fā)病及病變程度的相關性在线上看A片。

Calnexin and calreticulin are generally known as chaperons located in the ER. However, they are also involved in multiple functions other than chaperons.

calnexin 和calreticulin 一般廣為人知的功用，是位於內(nèi)質(zhì)網(wǎng)中扮演新生蛋白質(zhì)的保護子欧洲一级高潮喷水A片，幫助蛋白質(zhì)正確地進行折疊和糖基化王色一级毛片免费看。

Microglobulin modified with advanced glycation end products has been demonstrated to be a major constituent of amyloid fibrils.

DRA淀粉樣沉積物中的主要活性成份是晚期糖基化終產(chǎn)物修飾的〓微球蛋白，它具有廣泛的生物學活性。