However, commercially available CPB purified from porcine pancreas was very expensive, and was not totally free of other proteases, such as trypsin and chymotrypsin.

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In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

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The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根據(jù)氨基酸自動(dòng)分析儀測(cè)定的氨基酸組成和精確分子質(zhì)量計(jì)算出NSPC分子總共有331個(gè)氨基酸殘基香港一级婬片A片AA，分別是由40個(gè)Asp、22個(gè)Thr日韩精品在线看、18個(gè)Ser毛片毛片毛片高清免费、22個(gè)Glu、1個(gè)Gly国产18禁又污又黄又刺激、31個(gè)Ala中文无码亚洲色偷偷A片、27個(gè)Cys、17個(gè)Val91情侣在线精品国产免费、13個(gè)Ile欧美不卡一区、22個(gè)Leu、5個(gè)Tyr色呦呦在线播放、7個(gè)Phe在线观看a片免费网站、7個(gè)Lys、24個(gè)His色色的视屏、51個(gè)Arg黄色网址在线进、14個(gè)Prowww.樣、1個(gè)Met和9個(gè)Trp組成。胰蛋白水解酶分別酶解NSPC-1和NSPC-2后v亚洲色图欧美在线，分別采用MALDI-TOF-MS分析6969在线观看，其肽指紋譜圖顯示胰蛋白酶可將NSPC-1和NSPC-2水解成大小不等的多條肽段，質(zhì)量數(shù)在800以上的NSPC-2有11段国产精品v欧美精品v日本精品v，即水解的肽質(zhì)量分別為899.54脚交视频一区二区、1454.77、1544.9国产操穴视频、1683.87十大黄色免费网站、1803.93、1811.9118AV视频、1987.92免费看小姐操逼网站、2003.90、2536.26国产黄片亚洲欧美、2915.42和3492.72岛国免费人人操。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各種純化方法，將離子交換層析伊人色色网站、親和層析和疏水層析三種分離純化法相結(jié)合日韩黄色网站中文字幕，建立了激肽釋放酶、胰蛋白酶和糜蛋白酶三酶的聯(lián)產(chǎn)工藝：胰酶用pH4.5醋酸緩沖溶液提取后www.96，粗提液直接上DEAE-瓊脂糖快膠柱吸附平衡緩沖液：0.01mol／LNaoAc-HoAc緩沖液(pH4.5特黄大片AAAA级毛片，洗脫緩沖液：0.01mol／LNaoAc-HoAc緩沖液(pH4.5)含0.2～0.35mol／LNaCl分兩步洗脫，收集激肽釋放酶的洗脫峰日韩一级免费视频网站；DEAE-瓊脂糖快膠的未吸附液上親和層析柱分批吸附平衡緩沖液：0.01mol／LTris-HCl緩沖液(pH7.5亚洲免费播放片，洗脫液：0.5mol／LNaCl溶液，一次洗脫又粗又硬又大欧美69a√，收集胰蛋白酶洗脫峰黄视频网站观看地址点；最后，親和層析未吸附液用30％～80％硫酸銨分級(jí)鹽析處理欧美乱色伦图片区小说，沉淀溶解后用上苯基—瓊脂糖快膠吸附平衡緩沖液：0.01mol／LNaAc-HAc緩沖液(pH4.5含1mol／L(NH_4)_2SO_4十八岁免费网站，洗脫緩沖液：0.01mol／LNaAc-HAc緩沖液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分兩步洗脫全部免费A级毛片免费看，收集糜蛋白酶的洗脫峰看欧美性爱视频123区。

PACAP is a pleiotropic neuropeptide that belongs to the secretin/glucagon/vasoactive intestinal peptide family.

垂體腺苷酸環(huán)化酶激活肽是多效性的神經(jīng)肽，屬于腸促胰液素/胰高血糖素/腸血管活性肽家族免费观看黄色网站视频免费观看黄色。

We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.

采用氯乙趺琅破凳悠稻镁茫基保護(hù)天冬氨酸，然后進(jìn)行氨解生成GD二肽欧美黄色A片免费在线观看，利用豬胰脂肪酶催化合成RGD三肽国产精品大乳在线观看，著重解決了親水氨基酸/肽片段底物的溶解性與合成產(chǎn)率低的問題国产精品久久久久久亚洲按摩，建立了一種新的有效合成RGD三肽的反應(yīng)模型，并探索解決了極性較強(qiáng)的氨基酸的酶促肽合成的問題99er久久免费只有精品，對(duì)脂肪酶催化合成肽的影響因素從理論上作了較全面的分析，為肽片段的縮合反應(yīng)提供了一種新思路369超碰在线观看。

Methods : The levels of fasting blood glucose and fast plasma insulin, the activities of superoxide dismutase and glutathione peroxidase and level of lipid peroxidate were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice exposed to multiple stressors for 6 weeks. Results : The FBG level in STZ mice was elevated by stress, while the FINS level was not increased.

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Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路線使用α-胰凝乳蛋白酶和嗜熱桿菌蛋白酶完成了N-保護(hù)的亮-腦啡肽的酶法全合成，并在第三戊醇中首次以嗜熱桿菌蛋白酶為催化劑得到了Z-保護(hù)的亮-腦啡肽酰苯肼衍生物国产美女久久精品香蕉69。

The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.

用高效毛細(xì)管電泳測(cè)定了重組人促紅細(xì)胞生成素的微多相性久久综合无码一区丝袜、肽圖，同時(shí)采用毛細(xì)管電泳質(zhì)譜聯(lián)用技術(shù)鑒定了部分非糖基化胰酶消解片段和O－126糖基化肽的結(jié)構(gòu)亚洲无码在线天堂。

The results showed that: 1 SOD and GSH-PX activity of hepatopancreas and serum were significantly or extremely significantly increased when 5% or 10% alfalfa meal was added to the diet, whereas the MDA content of the two groups decreased. 2 The concentration of TC and TG in serum, hepatopancrac and muscle dropped as the amount of alfalfa meal increased.

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