METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

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Each subject's average monthly sulfur dioxides, nitrogen dioxide, ozone, carbon monoxide, particulate matter with aerodynamic diameter of less than10μm (PM10) exposure estimates during pregnancy were caculated.

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Delivered Ex-Quay – The seller is responsible for all costs involved in transporting the goods to the wharf at the port of destination.

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Chicago is one of the biggest climbers in the rankings since 2005, rising to 41st from 52nd due to reduced crime rates, while Cairo is one of the biggest fallers, sliding nine places to 131st out of 215 cities.

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The carrier gas is nitrogen, headspace plate temperature is 70℃.

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From the introduction of western medicine to inoculation of western pock, with the coming of Doctor Parker, these were the results of aids from the Hong merchants. Also the coming of Christianity had a close relationship with Thirteen Hongs.

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