Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文將對(duì)氧化還原狀態(tài)變化敏感的綠色熒光蛋白roGFP1-R12国产91最新第一，在酵母細(xì)胞中實(shí)現(xiàn)了多拷貝強(qiáng)表達(dá)；熒光掃描經(jīng)強(qiáng)氧化劑H_2O_2和還原劑DTT以及環(huán)境中重金屬NaAsO_2或Pb(NO_3)_2處理后的酵母細(xì)胞懸液另类综合80P，測(cè)定510 nm處的熒光發(fā)射強(qiáng)度結(jié)果顯示无码专区第一页中文字幕第一张，表達(dá)的綠色熒光蛋白對(duì)氧化還原水平敏感，且在510 nm處的熒光強(qiáng)度與一定的重金屬濃度呈正相關(guān)无码在线网址，即roGFP1-R12在510nm處的熒光發(fā)射值隨重金屬濃度的增高而增強(qiáng)美女一级黄色网站视频，從而說(shuō)明重金屬對(duì)細(xì)胞的毒性在一定程度上很可能是通過(guò)破壞細(xì)胞內(nèi)的氧化還原平衡發(fā)生作用；同時(shí)通過(guò)該綠色熒光蛋白對(duì)胞內(nèi)氧化還原狀態(tài)變化的響應(yīng)情況可以來(lái)實(shí)時(shí)檢測(cè)環(huán)境中的重金屬快射在线视频；遺傳學(xué)的點(diǎn)突變頻率及致死率實(shí)驗(yàn)數(shù)據(jù)表明人人澡人摸人人添av，重金屬能導(dǎo)致菌體的點(diǎn)突變頻率和致死率升高，且活性氧的清除劑巰基脲能明顯降低這種點(diǎn)突變和致死率欧美特黄大片，說(shuō)明由重金屬引發(fā)的這種點(diǎn)突變和致死效應(yīng)在很大程度上是依賴于重金屬對(duì)細(xì)胞誘導(dǎo)產(chǎn)生的氧化脅迫一区国产在。

Biological characteristics of the pathogen and the resistance of 13 cultivars of tea plant to tea brown blight were studied by means of natural infection in the field and artificial inoculation in vitro. Resistant cultivars and susceptible cultivars of tea plant were divided. And the resistance mechanism was researched tentatively. The findings are as follows: 1 Some biological characteristics of the pathogenic fungus The experiment result showed: the optimum temperature for the growth of fungus mycelium was from 24 to 27℃, the optimum temperature for sporulation ranged 22 to 30℃. Treated at 54℃ in 10 minutes, the spores didn't germinate. The optimum pH for the growth of the mycelium was 5.1～5.9, the optimum pH for sporulation ranged 5.0～6.0. Light had no much effect on the growth of fungus mycelium, but had much effect on the reproduction of the spore.

首先，研究了茶云紋葉枯病病菌的生物學(xué)特性欧美视频亚洲；其次亚洲精品在线观看视频免费，通過(guò)田間抗性調(diào)查和室內(nèi)人工接種鑒定，對(duì) 13 個(gè)茶樹(shù)品種進(jìn)行了抗病性鑒定人人草人人摸人人插；第三98国产视频，在前人研究的基礎(chǔ)上，對(duì)茶樹(shù)抗病機(jī)理作了更深入的研究午夜无码国产18禁在线香瓜社区，結(jié)果發(fā)現(xiàn)： 1 病原菌的生物學(xué)特性病原菌的分生孢子萌發(fā)的最適溫度范圍為 24～27℃亚洲黄色a片，菌絲生長(zhǎng)的最適溫度范圍為 22～30℃；致死溫度為 55℃人人操又人人；菌絲生長(zhǎng)的最適 pH 范圍為 5.1～5.991精品国产自产在线观看高清，孢子萌發(fā)的最適 pH 范圍是 5.0～6.0；光照對(duì)病菌生長(zhǎng)速度及分生孢子萌發(fā)影響不大精品久久久久久亚洲国产18，但對(duì)其產(chǎn)孢的影響很大国产亚洲日韩欧一级，特別是有光照與無(wú)光照處理之間差異顯著，光照有利于分生孢子的形成www人人插人人爱。