PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

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In the paper, processability of different proteins was investigated and the water retentiveness, emulsifiability and thermal stability of soybean separation protein, animal protein, lactalbumin, casein, and glutenin were compared.

研究了不同蛋白質(zhì)的加工性能色色啦啦，比較了大豆分離蛋白、動物蛋白欧美一区2区三区四区高清、乳清蛋白日韩欧美视频一区二区在现看、酪蛋白爱看AV在线、小麥蛋白的持水性、乳化性和熱穩(wěn)定性老女人操逼午夜视频。

The types of histone methyltransferases, the relationship between methylation of Lysine 9 of H3 and the formation of heterochromatin, gene regulation in euchromatin, and that with DNA methylation, were mainly introduced.

主要闡述了組蛋白甲基轉(zhuǎn)移酶的類型色哟哟在线高清播放，組蛋白H3中第9位賴氨酸甲基化與異染色質(zhì)的形成、常染色體中基因表達的調(diào)控黄色精品网，以及與DNA甲基化之間的關系AⅤ在线免费看，說明了組蛋白甲基化與組蛋白乙酰化黄片久久久、磷酸化的相互關系，指出組蛋白甲基化對維持細胞各種狀態(tài)的平衡起到極其重要的作用亚洲理论视频。

Pike protein consists of S1 and S2 subunit. S1 is head and S2 is rod-shape part, intermolecular effort can bind each other. S1 can bind with host cell epimembranal acceptor. S2 can fix integral Spike protein on the cellular membrane and mediate cell-fusion with virus and host cell. There are significant antigen determinant of virus in Spike protein, genic heterogenesis can effect viral pathogenicity. SARS-CoV Spike protein, which has 23 pieces larvaceous glycosylation sit, consists of 1256 amino acid. Its N-terminals and C-terminals is corresponding conservative.

蛋白是I型膜糖蛋白国产91网站在线，介導病毒與宿主細胞膜上的受體結(jié)合并與宿主細胞膜相融合。S蛋白由S1和S2兩個亞基組成国产激情一区二区三区app。S1形成球狀部分a片免费在线观看，S2形成棒狀部分，兩者之間通過分子間的作用力相互結(jié)合婷婷综合五月天丁香激情。S1能與宿主細胞上的受體結(jié)合jizz官网，S2可把整個S蛋白固定在細胞膜上，并介導病毒包膜與宿主細胞膜融合这男人看的黄色视频。成年人看的黄色视频。。S蛋白上有重要的病毒抗原決定簇玖玖日韩在线视频，S基因的突變會影響病毒的致病性。

Objective To explore livin, MTA1 and the caspase3 protein expression, their correlation and clinical pathology in colon cancer Methods The expressions of livin, MTA1 and caspase3 protein in 88 cases of colon cancer were detected by immunohistochemistry stainingResults Livin, caspase3 protein and MTA1 positive expression rates in colon cancer tissues were more than those in tissues beside the cancer Furthermore there were obvious relevance between livin protein, MTA1 positive expression rate and degree of histodifferentiation and lymph node metabasis and between caspase3 protein positive expression rate and degree of histodifferentiation of colon cancer Caspase3 protein expression had prominent inverse correlation with the livin expressionConclusions ①Over expression of livin having inhibtion on colon cancer is one of important factors of colon carcinogenesis ②Caspase3 protein expression in colon cancer tissues is inhibited to less and has prominent inverse correlation with livin expression Accordingly, suppressing caspase3 protein activity is one of channels, by which livin promotes colon carcinogenesis ③MTA1 plays the important role in histodifferentiation degree and lymph node metastasis of colon cancer

采用免疫組織化學染色方法檢測88例結(jié)腸癌組織中Livin射射人人，Caspase3 及MTA1的表達情況国产91页在线精品。結(jié)果（1）結(jié)腸癌組織中Livin的陽性表達率顯著高于癌旁組織，且Livin的表達與結(jié)腸癌的組織分化程度及淋巴結(jié)轉(zhuǎn)移程度顯著相關把女人弄爽A片视频。（2）結(jié)腸癌組織中Caspase3蛋白的陽性表達率顯著高于癌旁組織国产春黄视频网，且Caspase3蛋白的表達與結(jié)腸癌的組織分化程度顯著相關。（3）結(jié)腸癌組織中MTA1的陽性表達率顯著高于癌旁組織精品高清久久久久久久久，且MTA1的表達與結(jié)腸癌的組織分化程度及淋巴結(jié)轉(zhuǎn)移程度顯著相關中文字幕350页。（4）Caspase3蛋白的表達又與Livin的表達呈顯著負相關。結(jié)論（1）Livin在結(jié)腸癌組織中過表達久久精品视频九九，它可能是促進結(jié)腸癌發(fā)生的重要因素之一很黄很黄的免费视频在线观看。（2）Caspase3蛋白在結(jié)腸癌組織中表達被抑制而降低，且與Livin的表達呈負相關免费能直接看黄的网站视频直接看。因此成年人欧美片，抑制Caspase3蛋白的活性是Livin促進結(jié)腸癌發(fā)生的途徑之一。（3）MTA1對結(jié)腸癌的組織分化及淋巴轉(zhuǎn)移發(fā)揮重要作用操射网站。

Using SMARTTM cDNA library construction technology from Clontech biocompany,we have constructed 4 Matchmaker cDNA libraries for posterior silk gland of 4th moulting larvae,posterior silk gland,middle silk gland and fat body of larvae at 3rd day of 5th instar, respectively.

家蠶的絲腺是一個特異化的器官国产又粗又大又长免费视频，主要用于合成蠶絲蛋白日韩中文自拍无码AV，包括在家蠶的后部絲腺表達的絲素重鏈蛋白、絲素輕鏈蛋白那有毛片网站、P25蛋白(fibrohexamerin偷拍综合2区，F(xiàn)hx)以及在家蠶中部絲腺表達的絲膠蛋白。

Liver function and pathological changes of hepatic tissue were examined.

采用ANIT灌胃制備大鼠急性肝內(nèi)膽汁淤積病理模型日韩大陆一区二区三区在线观看AV，共32只大鼠一级片片片大A片，設正常組、正常＋大黃素組7777色综合、模型組和模型＋大黃素組二级免费黄片国产播放久久久,噜噜噜，觀察各組實驗動物肝功能各項生化指標和肝組織病理學改變，并采用實時熒光定量RT-PCR檢測肝細胞膜轉(zhuǎn)運蛋白基因多藥耐藥蛋白1a（mdr1a）国产在线播放线视频、多藥耐藥蛋白1b（mdr1b）和多藥耐藥蛋白2（mdr2）mRNA水平的表達国产特黄品爱网，Western blot方法檢測肝臟P-糖蛋白的變化。

The seed proteins and amino acids of legume seeds such as red bean,mung bean,pea and broad bean were studied by using the modernbiochemical technique,and the differences of quality in these proteinswere compared.

為比較食用豆類種子蛋白的品質(zhì)中文一级免费视频，我們對紅小豆国产无遮挡免费视频一区二区、綠豆、豌豆久草欧美、蠶豆的種子蛋白含量及氨基酸組成進行了生化分析人人干探花，并分別提取清蛋白、球蛋白日韩区精品在线、醇溶谷蛋白和谷蛋白老熟妇乱伦视频，測定其蛋白質(zhì)及氨基酸的含量。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通過分析可以將這些差異蛋白分為兩大類：第一類是調(diào)節(jié)性蛋白无码视频网站毛片一区二区，如咖啡酸三氧轉(zhuǎn)甲基酶欧美A片激情日，熱激蛋白70，醛酮變位酶I黄色免费在线观看网站，肌動蛋白等免费无删减大尺度漫画，它們主要參與蛋白質(zhì)的合成和加工、脅迫響應乱女伦露脸对白在线播放、細胞骨架穩(wěn)定等過程狼友综合；第二類是功能性蛋白，如抗壞血酸過氧化物酶五月丁香久久久，細胞質(zhì)蘋果酸脫氫酶一区二区黄色，細胞質(zhì)磷酸甘油酸激酶，S-腺苷蛋氨酸合成酶欧美一级片黄色黄片，ATP合酶等黄色网站日韩免费在线观看，它們則參與氧化還原平衡、光合作用新怡红院在线视频永久、光呼吸和各種代謝等過程亚洲中文精品图区。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

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