The researches show that the substrate plays an important role in the development of keratoconus. Cornea substrate's agenesis leads to the reduced solidity, which is possibly related to kearatoconus. There is still a lot to be researched on etilogy and early diagnosis of keratoconus.

雖有研究表明角膜間質(zhì)的水平在圓錐角膜的發(fā)病中起重要作用自拍高潮日韩在线观看网站大全视频，角膜間質(zhì)發(fā)育不全国产精品电影久久久久电影院，導(dǎo)致角膜的穩(wěn)固性下降18禁成年大片免费网站，可能與圓錐角膜形成有關(guān)，但對圓錐角膜形成的機(jī)理仍不詳盡亚洲一区二区三区在线看，因此中文字幕激情亚洲，對圓錐角膜的發(fā)病機(jī)制及早期診斷的研究具有重要意義。

Paired Samples T test revealed that both the LT and VL decreased (P.01), the AD increased (P.01) after cycloplegia regardless of their refractive state. The AL increased for hyperopic eyes (P.05) and decreased for myopic eyes (P.01) after cycloplegia. For hyperopic eyes, the major keratometer K1 increased (P.05) while for myopic eyes, the major keratometer K1(P.05) and minor keratometer K2 (P.01) decreased after cycloplegia. The mean corneal power of zones 5 mm (MD5) and 7 mm (MD7) decreased with myopic eyes decreased dramatically (P.01). The mean refractive power of the lower half vertical meridian is greater than that of upper half; and the mean refractive power of the lateral half of the horizontal is greater than that of the medial half.

結(jié)果：不論遠(yuǎn)視眼射,黄色视频、正視眼還是近視眼在睫狀肌麻痹后前房加深（P.01）欧美二区乱伦视频，晶狀體變薄（P.01）大学生粉嫩12p国产，玻璃體腔徑縮短（P.01）制服丝袜人妻日韩在线；但遠(yuǎn)視眼睫狀肌麻痹后眼軸增長（P.05），近視眼眼軸縮短（P.01）深夜特黄A级毛片免费视频,；遠(yuǎn)視眼睫狀肌麻痹后角膜曲率K1增加（P.05）可以直接在线观看黄网站免费，近視眼睫狀肌麻痹后除K1降低外（P.05），K2以及以角膜中心點(diǎn)為中心99久热只有精品视频最新，直徑為5mm美女裸体日韩成人网站视频在线观看、7mm的環(huán)平均角膜屈光度（MD5、MD7）均降低（P.01）在线观看无码区；此外免费在线观看黄片AV，垂直徑線上方的平均角膜屈光度小于下方的平均角膜屈光度而水平徑線鼻側(cè)的平均角膜屈光度小于顳側(cè)的平均角膜屈光度（P.01）。

The results of corneal topography, that of keratometer and cycloplegic retinoscopy in measuring astigmatism before refractive surgery were compared, and the measuring results of corneal topography and that of keratometer for 72 eyes after photorefractive keratectomy and 95 eyes after laser in situ keratomileusis were also compared.

采用計(jì)算機(jī)輔助的角膜地形圖九一精品视频在线美女在线观看、角膜曲率計(jì)及散瞳驗(yàn)光三種測量散光的方法国产精品久久久精品日日，對屈光不正患者360只眼進(jìn)行檢查，同時(shí)對準(zhǔn)分子激光角膜切削術(shù)（photorefractive keratectomy欧美激情丁香综合，PRK）后72只眼和準(zhǔn)分子激光原位角膜磨鑲術(shù)（excimer laser in situ keratomileusis日批视频网站免费看，LASIK）后95只眼的角膜散光用角膜地形圖和角膜曲率計(jì)進(jìn)行測量并比較。

Thirty-two pathological corneal samples were collected from the patients during corneal transplantation, including keratoconus (3 cases), endothelial keratitis (1 case), corneal endothelial decompensation after cataract operation (4 cases), Mooren's ulcer (3 cases), corneal implant opacities (5 cases), herpes simplex viral leucoma (1 case) and perforation (3 cases), trachoma corneal leucoma (1 case), bacterial (1 case) and fungal (1 case) corneal perforation, chemical trauma (5 cases), and burn (2 cases).

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A corneal abrasion with a diameter of 7 mm was created centrally in both eyes with a trephine and subsequent rubbing off on the corneal epithelium with a 20% alcohol soaked swab. Photographic documentation and hematoxylin and eosin stained pathological section were performed when rabbits were killed at 0,12,24,36,48 and 72 h. Planimetry was performed, and the corneal photographs were analyzed with computer software.

將4組兔雙眼角膜中央置入直徑7.00mm的環(huán)鉆呦呦色色网，其內(nèi)滴入20%乙醇約60s后用角膜刮匙刮去角膜上皮亚洲无线码在线一区观看，制作角膜上皮缺損模型，術(shù)后每組滴用眼液頻率均為3次／d天天色天天爽，各組分別于術(shù)后12一级片免费观看，24，36www.色色.com，48及72h行裂隙燈下熒光素染色照相及海德堡共焦激光角膜顯微鏡檢查后各處死2只兔无码有码日韩制服AV，取角膜做病理切片同時(shí)應(yīng)用計(jì)算機(jī)圖像處理系統(tǒng)測量角膜損傷后愈合面積。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基質(zhì)瓣91自拍偷拍第一页；轉(zhuǎn)染組用含rAAV－EGFP轉(zhuǎn)染液浸泡角膜瓣和角膜基質(zhì)床10 min日韩人人操人人射，對照組用等量BSS液浸泡角膜瓣和角膜基質(zhì)床10 min，不同時(shí)間點(diǎn)(1黑人与黑人作爱A级毛片、3老熟妇一区二区免费视频、7、14人妻欧美精品片在线97、21d)通過熒光體視鏡觀察角膜出現(xiàn)EGFP熒光的起始時(shí)間及分布情況www。五月天。com，收集角膜，其中一半用激光共聚焦顯微鏡觀察久久国产精品亭亭亚洲Av、照相两个人的黄色网站，另一半固定后行HE染色。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

實(shí)驗(yàn)組經(jīng)器官培養(yǎng)保存4周后以角膜測厚儀測量角膜厚度亚洲免费性爱片一级，然后每個(gè)角膜被分成兩半亚洲a级黄片免费观看，共48個(gè)半片角膜，再分成4組美女被操网站在线播放，每組12個(gè)半片欧美久久久久精品一区。12個(gè)半片用茜素紅-臺盼藍(lán)染色染色行角膜內(nèi)皮細(xì)胞計(jì)數(shù)；12個(gè)半片角膜用4%中性福爾馬林溶液固定行HE染色先锋影音免费资源站、應(yīng)用免疫組化染色檢測AQP-1在角膜基質(zhì)和內(nèi)皮細(xì)胞表達(dá)的改變一级大黄片操逼的；12個(gè)半片角膜用Na~+-K~+-ATP酶試劑盒測量角膜內(nèi)皮細(xì)胞Na~+-K~+-ATP酶活性；12個(gè)半片角膜用實(shí)時(shí)熒光定量PCR檢測AQP-1mRNA表達(dá)改變国产专区在线操。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

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MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

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The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

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