In this study, we isolate, purify and characterize endo-β-glucanase by Ascomycete Xylaria regalis. The cellulose-containing medium is used to induce the production of the cellulose-degrading enzymes. When these enzymes are produced at maximum level, we harvest and filtrate the culture medium. The filtrate is purified by anion exchanger column and hydrophobic interaction column (phenyl-650M). The purification fold of endo-β- glucanase increases 4.3 times, the specific activity approaches 3.7 unit/mg, and the recovery is 27.6%, respectively.

為了分離且純化此株菌產(chǎn)生在培養(yǎng)基中的endo-β-glucanase，先將此菌在cellulose液態(tài)培養(yǎng)基中大量培養(yǎng)亚洲日韩国产高清mp4，在此酵素達(dá)到最高峰時(shí)免费欧美日韩一区二区视频，利用濾紙過(guò)濾將菌絲及菌液分開(kāi)，收集粗酵素液99h久久久精品，再經(jīng)過(guò)陰離子交換樹(shù)脂及疏水性層析管柱(Phenyl-650M)等步驟予以純化91超碰在线播放，所得到最終酵素純度為 4.3倍美女黄污视频在线播放，比活性為3.7 unit/mg，回收率為27.6%日日做夜夜操。

Intestinal juice contains all food enzymes (carbohydrase, protease and lipase) for complete digestion of food.

小腸液含有所有食物酵素(碳水化合物酵素全免费一级毛片免费看在线、蛋白質(zhì)酵素和脂肪酵素)，可完成食物的消化天堂婷婷久久。

Contain enzymes (carbohydrase, protease and lipase) which digest carbohydrates, proteins and fats.

含有可消化碳水化合物，蛋白質(zhì)和脂脂的酵素碳水化合物酵素上海黄色录像免费一级，蛋白質(zhì)酵素和

In clinical. acetylcholinesterase inhibitors reduce the breakdown of synaptic acetylcholine, have been modestly effective in improving the cognitive deficits of Alzheimer's disease.The goal of this work is to determine enzyme kinetics and mechanisms of acetylcholinesterase and butyrlcholinesterase inhibition by five cardiovascular drugs, lovastatin, simvastatin, amlodipine besylate, nifedipine, and hydralazine hydrochloride, and two benzodiazepines, diazepam and chlordiazepoxide hydrochloride. All drugs in this study are reversible mixed-type inhibitors of acetylcholinesterase and butyrylcholinesterase. The pKi values for acetylcholinesterase and butyrylcholinesterase inhibition by the cardiovascular drugs are linearly correlated with the molecular weights of the drugs with the slopes of 0.005 and 0.0021, respectively. Therefore, van der Waals' interactions between acetylcholinesterase and the cardiovascular drugs are stronger than those between butyrylcholinesterase and the drugs. This is probably due to a smaller active site gorge and a more significant peripheral anionic substrate binding site of acetylcholinesterase than those of butyrylcholinesterase.

本研究之目的系決定乙醯膽堿酯酵素和丁醯膽堿酯酵素被五種心臟血管藥物lovastatin人人操人人插人人射人人人， simvastatin， amlodipine besylate国产黄网站在线免费观看， nifedipine欧美一级AA大片免费观看、hydralazine hydrochloride和兩種benzodiazepines：diazepam和Chlordiazepoxide hydrochloride的抑制作用之動(dòng)力學(xué)及機(jī)轉(zhuǎn)，這些藥物都是乙醯膽堿酯酵素和丁醯膽堿酯酵素的可逆性91最新网站免费、混合型之抑制劑欧美在线黄片小视频，實(shí)驗(yàn)結(jié)果顯示，五種心血管藥物對(duì)於乙醯膽堿酯酵素及丁醯膽堿酯酵素抑制之pKi值和藥物之分子量呈直線(xiàn)之關(guān)系欧美第一区在线，斜率分別為0.005及0.0021因此丁醯膽堿酯酵素你懂的的网站，與心血管藥物間之凡德瓦作用（van der Waals' ）比乙醯膽堿酯酵素與心血管藥物間者弱，這可能原因是丁醯膽堿酯酵素之活性區(qū)域比乙醯膽堿酯酵素之活性區(qū)域更寬廣黄片动漫在线观看，但丁醯膽堿酯酵素之周邊陰離子區(qū)域不及乙醯膽堿酯酵素之周邊陰離子區(qū)域者明顯重要www欧美，由於五種心血管藥物對(duì)於乙醯膽堿酯酵素和丁醯膽堿酯酵素抑制之pKi值存在著線(xiàn)性關(guān)系表示此種抑制作用系經(jīng)過(guò)共同之反應(yīng)機(jī)理。

Bamboo mosaic virus encodes a 155-kDa replicase that the middle region contains a helicase-like domain according to the existence of several conserved featured motifs of helicase. Previous studies showed that the helicase-like domain exhibits both ATPase and RNA 5'-triphosphotase activities, and Mg2+ is essential for both activities. Mutagenesis of conserved motifs showed these amino acids play important roles for enzymatic activities. These results suggested that the properties of this denatured and renatured protein are coincident with other SF1 helicases.

從胺基酸序列分析顯示全免费a级毛片不卡，竹嵌紋病毒復(fù)制酵素中間區(qū)域的類(lèi)解螺旋酵素具有解螺旋酵素的保留性胺基酸區(qū)域亚洲国产精品永久在线，經(jīng)實(shí)驗(yàn)顯示此蛋白具有ATPase和RNA 5'-triphosphatase活性，而且是依賴(lài)鎂離子進(jìn)行反應(yīng)的亚洲无码视频亚洲人的网站，其中保留性胺基酸的突變會(huì)破壞其催化能力操学生妹视频在线观看，說(shuō)明這在大腸桿菌表達(dá)的蛋白質(zhì)經(jīng)過(guò)變性及重新摺疊的過(guò)程，具有和其他SF 1解螺旋酵素相似的特性高h一区。

Collagenase activity was defined as the radioactivity extractable by 50% dioxane. Clostridial collagenase was used as a standard. The lower limit of this assay method was 0.01 units of clostridial collagenase. Among 17 glaucoma eyes, 6 had trace collagenase activity (around 0.01 units) in aqueous humor; and for cataract eyes, 8 out of 27 had trace collagenase acitivty.

我們所采用之測(cè)量方法可偵測(cè)出約0.01單位以上的膠原酵素日日插日日操；一般前房液之膠原酵含量非常低，許多患者無(wú)法以我們之方法利得膠原酵素之存在：17位青光眼患者中一级毛片网推荐，只有6位可於其前房液中到得約0.01單往之膠原酵素日韩午夜丝袜无码视频；27位白內(nèi)障急者中，有8位可測(cè)得微量膠原酵素夜夜人人操；二者在統(tǒng)計(jì)學(xué)上與太大差異存在国内精品一三级成年视频在线观看。

These ferments are made of the roofs, leaves and stems of 58 handpicked kinds of plant and vegetables through exclusive the biochemical technology, with the high-reactivity, high-stability and high-safety; The second is the fruit compound ferments(the approval No.J11000510836) with the abundant vitamin, minerals, favorable viable groups, multi-amino acid groups, oligosaccha groups and glucose , is made from handpicked 103 kinds of fruits and 20 kinds of Chinese herbal medicines through microbial treatment and fermentation method; The third is grain and bean high-fiber nutritious ferments (the approval No.J11000600369) with abundant proteases, amyloclast enzyme and lipolytic enzyme, is made from 60 kinds of grains and beans through special biologic technology, and is used to lose weight, expel toxin, clear the bowels, supply nutrition and activate cells in human body.

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In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本實(shí)驗(yàn)欲進(jìn)一步探討加熱處理之抱子甘藍(lán)對(duì)phase Ⅰ與phase Ⅱ解毒酵素活性之影響差異，實(shí)驗(yàn)分三組性a免费视频\，抱子甘藍(lán)(1)冷凍乾燥組（glucosinolate未裂解）(2)加熱冷凍乾燥組(3)半量之裂解冷凍乾燥組（glucosinolate已裂解）欧美日韩www.59要艹，(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成飼料，餵以小白鼠（4只／組）7天717午夜福利视频网，結(jié)果發(fā)現(xiàn)国产AV天天更新，在第(2)組小白鼠GST phase Ⅱ酵素）活性顯著上升，顯示在植物裂解酵素不存在之狀況下久久这里精品视频免费3，腸胃道之微生物仍可促進(jìn)裂解反應(yīng)黄色片欧美性爱，但由CYP1A活性增加不顯著（僅1.4倍）看來(lái)，根據(jù)定義：?jiǎn)为?dú)phase Ⅱ酵素活性增加自慰网站污污，為monofunctional induction/inducer三级性爱视频免费在线观看，可加速致癌物之代謝。

Cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A.

接著并以活性為導(dǎo)向的分離策略性欧美久久，從樟芝子實(shí)體乙醇抽出物中分離出具抗發(fā)炎活性成分antrocamphin A好看黄色视频，續(xù)利用LPS誘導(dǎo)小鼠巨噬細(xì)胞（RAW 264.7）產(chǎn)生發(fā)炎的模式人人射人人摸人人草人人操人人射，解析antrocamphin A之抗發(fā)炎機(jī)制，結(jié)果證實(shí)antrocamphin A確可有效抑制一氧化氮自由基和前列腺素的生成色网站五月天，透過(guò)蛋白質(zhì)表現(xiàn)分析得知91狠狠综合久久久久精品网站，antrocamphin A之抗發(fā)炎活性是經(jīng)由抑制一氧化氮生成酵素和第二型環(huán)氧酵素的mRNA表現(xiàn)，進(jìn)而抑制了一氧化氮生成酵素国内自拍第一页91视频、第二型環(huán)氧酵素和核轉(zhuǎn)錄因子-κB等酵素之表現(xiàn)欧美日韩中文字幕99久。

Lipases and chitinases are industrially useful enzymes, and have the potential applications for use in food industry.

脂質(zhì)酵素、磷脂質(zhì)酵素及幾丁質(zhì)分解酵素是工業(yè)上應(yīng)用研究近年來(lái)倍受矚目的重要酵素日本特级黄大片一区二区三区，其在食品工業(yè)上應(yīng)用的潛力亦大老牛影视无码av一区。