The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,300 IU/g of KDN pectinase,35℃免费看的黄色视频、6 h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 500 IU/g of TZ-888 compound enzyme,45 ℃,6 h.

摘 要：本研究根據(jù)苧麻膠質的主要成分為果膠人人操人人爱一区二区、半纖維素和木質素免费专区-iGAO视频网_为爱搞点激情，用高活性的KDN果膠酶和TZ-888復合酶（主要是木聚糖酶和甘露聚糖酶）從pH值、浴比日韩激情中文字幕在线看、酶用量、金屬離子日韩av精品视频在线播放、溫度九九精选视频、脫膠時間等方面進行單因素試驗，然后選取影響顯著的浴比国内品精久久久久久久国产、酶用量、溫度91三级片在线视频观看，時間進行四因素三水平的脫膠試驗一区二区亚洲，結果表明鮮麻采用KDN果膠酶脫膠最佳組合為浴比1：18，pH 8.6一级黄色视频高潮， 1 mmoL/L Mg2+国产一级视频免费，酶用量300 IU/g，溫度35 ℃亚洲,日韩,欧美小说视频、時間6 h時處理免费咸人AAAAAAA毛片，TZ-888復合酶脫膠最佳組為浴比1：12，pH 4.0人人插人人做人人干， 1 mmoL/L Ca2+十八级毛片在线观看99ri，酶用量500 IU/g，溫度45 ℃五月丁香激情久久，時間6 h進行脫膠為優(yōu)化的試驗條件日韩在视频。

The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on dry ramie degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,20 IU/g of KDN pectinase,4℃、4h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 300 IU/g of TZ-888 compound enzyme,40 ℃,5 h.

摘 要：本研究根據(jù)苧麻膠質的主要成分為果膠免费黄色视频应用、半纖維素和木質素黄色页一级在线观看，用高活性的KDN果膠酶和TZ-888復合酶（主要是木聚糖酶和甘露聚糖酶）從pH值、浴比亚洲二级激情aaaa片、酶用量精品163黄色录像、金屬離子、溫度99r在线精品视频播放、脫膠時間等方面進行單因素試驗欧美视频在线观看免费电影，然后選取影響顯著的浴比、酶用量抠屄操b视频、溫度欧美一级作爱牲交片免费观看，時間進行四因素三水平的脫膠試驗，結果表明干苧麻采用KDN果膠酶脫膠最佳組合為浴比1：12欧美影院a∨天堂，pH 8.6手机在线视频中文字幕精品三上， 1 mmoL/L Mg2+，酶用量200 IU/g搜毛片索毛片搜黄片搜黄片色毛片，溫度45 ℃污视频网站国产免费、時間4 h時處理日韩无码h，TZ-888復合酶脫膠最佳組為浴比1：18，pH 4.0男女18禁啪啪无遮挡激烈动态图红， 1 mmoL/L Ca2+免费成年人在线，酶用量300 IU/g，溫度40 ℃人人澡人人看人人摸，時間5h進行脫膠為優(yōu)化的試驗條件国产综合男女视频一区二区。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我們根據(jù)在脲或鹽酸胍存在下的底物反應動力學方程求得游離酶和酶底物二元99农村一级毛片、三元復合物的微觀動力學常數(shù)日韩一区黄色小视频，結果表明：①酶-底物二元、三元復合物的失活速度明顯慢于游離酶黄网站色视频免费一级毛片，說明兩個底物二氫葉酸和NADPH對酶的失活都具有一定程度的保護作用日韩-本道黄色网站站；②在脲作用下，酶的失活和構象變化均為單指數(shù)項過程国产又黄又爽又色视频在线，而且酶的活力喪失和三級結構變化是同時發(fā)生的久久免费AV片国产精品，說明二氫葉酸還原酶的脲變性可能是一個"全或無"的兩態(tài)過程；③在鹽酸胍作用下熟妇操B，酶的構象變化為兩相過程日本高清wwwcom，而失活則是單指數(shù)項過程，酶分子構象變化的快相速度常數(shù)與失活速度常數(shù)基本一致中文自拍日韩视频在线观看，因此我們認為二氫葉酸還原酶的鹽酸胍變性過程中存在一個沒有活力免费看无码视频进入口、但仍具備一定空間結構的變性中間體。

In April 2004, 30 Boleophthalmus pectinirostris samples were collected from the lagune in Xiepu Villege, Zhenhai Town, Ningbo City, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in liver tissue. LDH, FDH, GDH, GCDH, G6PDH, MDH, ME, SOD, CAT and EST were analyzed and the biochemical genetic results showed that, among the 10 isozymes in this paper, 9 of which obtained clear enzyme patterns except for CAT.

于2004年4月丁香花在线观看视频在线，在浙江省寧波市鎮(zhèn)海區(qū)澥浦灘涂采集野生大彈涂魚30尾午夜性色一区二区免费视频，采用聚丙烯酰胺凝膠電泳方法，分析了乳酸脫氫酶一级国产、甲酸脫氫酶全免费毛片免费看、谷氨酸脫氫酶、D-葡糖脫氫酶欧美一级黄色大片网址、葡萄糖－6-磷酸脫氫酶(G6PDH)美女露内裤扒开腿视频在线观看、蘋果酸脫氫酶、蘋果酸酶黃色A片一级一级一级、超氧化物歧化酶97人人爽人人添天天爽、過氧化氫酶和酯酶等10種酶在大彈涂魚肝臟組織中的表達，并對同工酶表型進行了生化遺傳分析日本三级视频在线观看。

The results of isozyme analysis show that the number of isozyme bands of peroxidase, cytochrome oxidase and superoxide dismutase are 13, 11 and 10, respectively. But that of amylase, esterase and malate dehydrogenase are only 4, 7 and 7, respectively.

結果表明：過氧化物酶欧美黄色网站在线看、細胞色素氧化酶、超氧化物歧化酶等三種酶的同工酶酶譜很豐富欧美精品一色喲哟，分別有13你懂得视频网站、11久久国产免费一区二区三区、10條酶帶；淀粉酶一级一级a爱片免费免免、酯酶欧美黄片1区,2区,3区、蘋果酸脫氫酶等三種酶的同工酶酶帶相對較少，分別為4欧美日韩成年人色免费看片、7一级黄色在线免费看、7條。

The biocatalyzed characters of the purified cellulases were investigated with the CMC-Na as substrate. The most appropriate catalyzed temprature for cellulase 1 is 60 "C,and 65 for cellulase 2. The two enzymes have the extreme stability at the temperatures no more than 50.The CMC-Na has the protective effect on the cellulase. The pH stability range of cellulase 1 is 4 ~ 8, and 6-9 for cellulase 2. Zn2+, Ca2+, Mg2+, K+, Li+can ativate cellulase 1. K+, Li"1 can ativate cellulase 2. Cellulase 1 can mainly decompose CMC and salicin. Cellulase 2 can decompose not only CMC and salicin, but other substrates feebly.

以羧甲基纖維素為底物時永久看欧洲大片，酶1的最適催化溫度為60℃av中文在线一级片，酶2的最適催化溫度為65℃，在50℃以下穩(wěn)定性較好欧美激情亚洲日韩专区，底物對酶有較強的保護作用；酶1和酶2的最適pH分別為5.5和5.5～6.0一区二区视频在线导航，酶1的pH穩(wěn)定性范圍為4～8成人精品视频在线日韩免费观看网站，酶2的穩(wěn)定性范圍為6～9；Zn~(2+)老熟女交性毛片视频，Ca~(2+)六六色色，Mg~(2+)，K~+亚洲天堂综合网，Li~+對酶1有激活作用亚洲精品色色色，K~+，Li~+對酶2有激活作用播放国产精品；酶1主要對CMC和水楊素有分解作用无码不卡乱人伦中文视频，對其它底物幾乎不分解；酶2除了可以分解CMC和水楊素外欧美老年黄片，對其它底物也有微弱的分解作用jizz免费在线国产。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后確認：股骨與骨質疏松發(fā)病可能相關的蛋白6個，分別為：乳鐵蛋白輕鏈免费观看黄视频网站、膜聯(lián)蛋白A3国内自拍网、烯醇化酶、ATP合酶午夜性大片免费视频、乙酰輔酶A還原酶午夜福利试看120秒体验区、肌鈣蛋白；腰椎與骨質疏松發(fā)病可能相關的蛋白5個色a电影网，分別為：肌動蛋白久久性网、角蛋白、烯醇化酶美女视频黄视频、ATP合酶欧美在线观看精品一区二区三区、肌球蛋白亚洲资源一区二区在线观看；股骨與強骨寶療效可能相關的蛋白9個，分別為：烯醇化酶性欧美裸体XⅩⅩ、ATP合酶精品国产国语对白AⅤ、肌鈣蛋白、肌酸激酶同工酶黃色視頻免費觀看網址、磷酸甘油酸變味酶国产一区精品自拍、肌球蛋白、乳鐵蛋白輕鏈欧美日韩国产专区、丙酮酸激酶同工酶欧美日韩二区视频、冠蛋白；腰椎與強骨寶療效可能相關的蛋白8個绿帽社区，分別為：碳酸酐酶日本AAA片、肌動蛋白、αB-晶體蛋白国产精品中文字幕丝袜制服强奸乱伦、3-磷酸甘油醛脫氫酶毛片无码高清、血清白蛋白、ATP合酶欧美一区精品有色视频、肌球蛋白日韩高清在线一区二区三区、烯醇化酶。

Although the 脲酶 activity of soil is one of impact factor that the ammonia vaporizes, don't have obvious relativity.

雖然土壤的脲酶活性是氨揮發(fā)的影響因素之一www.99热，但不具有明顯的相關性色一级A片视频在线播放网站大全。

Both of the venoms showed activities of multiplehydrolases including hyaluronidase, protease, DNase, acid phosphetase,alkaline phosphetase and choline esterase, of which hyaluronidase, protease and DNase were more active. The activity of protease in VDPGwas higher than that of protease in VES and the activities of the resthydrolases in VDPG were lower than those of hydrolases in VES.

通過對粗毒進行酶活性分析，發(fā)現(xiàn)兩種粗毒都具有透明質酸酶九九视频精品在线、蛋白水解酶欧美性爱乱、DNA酶、酸性磷酸酶26uuu亚洲日韩欧美、堿性磷酸酶91无码精品xyz、乙酰膽堿酯酶等酶活性；其中透明質酸酶在线观看黄A片免费网站免费、蛋白水解酶黄色轻点疼视频网站、DNA酶活性較高；毒囊粗毒中蛋白水解酶活性高于電刺激粗毒好爽好舒服要高潮了视频在线观看，而其它幾種酶活性均低于電刺激粗毒亚洲影院在线观看播放。

He analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L^(-1) TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase).

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