NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通過研究，我們提出了初步的NodD操縱子激活模型：第一hytkb.com，四聚體是NodD蛋白的功能單位韩国精品视频在线一区二区，它通過鉚釘兩個串聯(lián)的相對獨(dú)立的DNA靶位點(diǎn)結(jié)合被誘導(dǎo)的啟動子；第二色哟哟影视基地，小分子配基的結(jié)合是改變NodD四聚體的構(gòu)象而不是引發(fā)不同形式的寡聚體a片在线观看哟哟哟，在我們的模型中，NodD四聚體縮小其V形主體的彎折角456小视频，進(jìn)而縮短其DNA結(jié)合功能域的間距69免费视频；第三，小分子信號的誘導(dǎo)并沒有改變NodD的DNA結(jié)合域和其DNA靶位點(diǎn)的相互作用日韩手机福利写真在线观看，NodD的構(gòu)象改變由蛋白質(zhì)經(jīng)其雙鉚釘位點(diǎn)傳遞給DNA日本三级欧美三级人妇视频；第四，只有誘導(dǎo)狀態(tài)的NodD激發(fā)的DNA構(gòu)象才能有效地使RNA聚合酶形成轉(zhuǎn)錄開放復(fù)合物污污色色；第五视色4se，不排除NodD與RNA聚合酶可能有直接的相互接觸位點(diǎn)，不排除NodD構(gòu)象的改變可能直接有利于RNA聚合酶形成轉(zhuǎn)錄開放復(fù)合物高潮网址，但是我們認(rèn)為NodD構(gòu)象改變本身不是充當(dāng)轉(zhuǎn)錄激活開關(guān)的決定因素99热这里。

Esterases and lipases have been widely applied in various industrial process and thermophilic esterases have a great potential application for their thermo-stability.

中文摘要酯酶和脂肪酶已經(jīng)被廣泛的應(yīng)用于多種工業(yè)中，嗜高溫酯酶因優(yōu)良的熱穩(wěn)定性有很大的應(yīng)用潛力人人上人人肉人人插。

It is implied that research on thermostable chitinase is of importance in industrial exploitation.

但市場應(yīng)用的幾丁質(zhì)酶主要來源于常溫菌三级黄色视屏，因產(chǎn)品成本高，熱穩(wěn)定性差欧美18xxoo，貨架壽命短www.baoru.com，酶活力和產(chǎn)率低而制約幾丁質(zhì)酶在農(nóng)業(yè)、工業(yè)上的應(yīng)用黄色网站大全入口。

The results showed that granular urea amended with dicyandiamide and N- thiophosphoric triamide induced the highest soil nitrate-reductase activity, granular urea brought about the highest soil urease activity and microbial biomass C a...

結(jié)果表明亚洲色久悠悠A∨明星换脸，施用硝化抑制劑和脲酶抑制劑涂層大顆粒尿素肥料的土壤硝酸還原酶活性最高；施用大顆粒尿素天天摸人人操人人爱，脲酶活性最強(qiáng)又粗又大特黄片，微生物量碳、氮最高附近那有黄色A片。

The results showed that 22.5μL/L of clove leaf oil had the best preservation effect on peach. After 35 days of stock, the sound fruit rate was 94%, 46.9% higher than compared group. The rotten index and the browning index were 94.1% and 85.2% lower than the control, respectivly, and the treatment could remarkablely reduce the respiration rate and ethylene release, and postponed ethylene release peak and respiration peak 5 and 10d, respectively in comparison with CK, it also delayed the drop of firmness and contents of titrable acids of the peach. When stored for 35d, firmness of control group and 22.5μL/L of clove leaf oil were decreased 60% and 34% compared with initial storage, and the activities of SOD, CAT and POD were enhanced, but the contents of SSC had no obvious difference with control.

結(jié)果表明天天色综合av，與對照相比国产看黄一区，22.5μL/L丁香葉油處理對桃果實(shí)的防腐保鮮效果最好精品激情，貯藏35d時(shí)好果率為94%看久久久久久久久特级毛片，比對照提高了46.9%，腐爛指數(shù)和褐變指數(shù)分別比對照低94.1%和85.2%日韩国产一区；丁香葉油處理可以有效降低桃果實(shí)的呼吸強(qiáng)度和乙烯釋放量www.slgxq.com，呼吸強(qiáng)度高峰和乙烯釋放量高峰分別推遲5和10d人人射AV网站，延緩了果實(shí)硬度和可滴定酸含量下降的速度；貯藏35d時(shí)對照和22.5μL/L丁香葉油處理果實(shí)硬度分別比貯藏初期降低了60%和34%日韩视频在线免费播放；丁香葉油處理提高了超氧化物歧化酶欧美加勒比、過氧化氫酶、過氧化物酶3種膜脂過氧化物酶的活性操亚洲无码视频，但可溶性固形物含量與對照相比無明顯差異xxxx.wwww。

The toxical effects of nonylphenol ethoxylates and nonyiphenol were studied with EROD in liver of carp as biomarker under external condition. These two tested substances all had activation on EROD enzyme in liver of carp. Under definite range of concentrations, with the affecting rule of inhibiting at high concentration and inducing at low concentration.

在離體條件下，以鯉魚肝臟EROD酶為生物標(biāo)志物研究了壬基酚聚氧乙烯醚及壬基酚的毒性效應(yīng)結(jié)果表明色综合网久久，在一定的濃度范圍內(nèi)，2種受試物對鯉魚肝臟EROD酶均有激活作用受試物濃度對EROD酶的活性的影響規(guī)律為高濃度時(shí)抑制人人操天天操老太大逼人人操，低濃度時(shí)誘導(dǎo)免费高清无码在线视频。

The dynamic variation of laccase activity and pH were detected during fermentation of Trametes versicolor 1126. The effects of sodium carboxymethyl cellulose and phenol on laccase activity were also studied.

考察了云芝Trametes versicolor 1126發(fā)酵培養(yǎng)中漆酶酶活和pH的變化，同時(shí)研究了羧甲基纖維素鈉及苯酚添加量對漆酶活力的影響一片黄网站。

Trametes trogii was screened from Majia Hill in Weihai. The effect of pH, temperature and inorganic ion on Trametes trogii xylanase activity and stability was studied. Meanwhile, the substrate concentration effect of this xylanase was also studied and Km was measured.

從威海市瑪珈山上獲得毛栓菌800av无码，在對其產(chǎn)酶條件研究的基礎(chǔ)上，主要研究pH国产区影院、溫度亚洲专区欧美专区日韩专区另类、無機(jī)離子對毛栓菌木聚糖酶活性和穩(wěn)定性的影響，以及該酶底物濃度效應(yīng)和Km值測定日韩欧美一。

To determine the role of neutral trehalase during storage stability of spores, we constructed a M.anisopliae RNAi transformant and an overexpression transformant. Analysis of these transformants revealed that the expression level of the neutral trehalase gene in RNAi transformant(No 688) was reduced to 50% of which found in wild type and the overexpression transformant(No 113) showed approximatlely 2-fold higher levels of neutral trehalase mRNA than that found in wild-type control.

構(gòu)建了中性海藻糖酶基因RNA干擾載體和超表達(dá)載體人人人人干丝袜，采用電轉(zhuǎn)法和基因槍法轉(zhuǎn)化金龜子綠僵菌CQMa102，獲得了RNA干擾轉(zhuǎn)化子和超表達(dá)轉(zhuǎn)化子99网一级A片在线免费播放，比較這兩種轉(zhuǎn)化子和出發(fā)菌株的mRNA表達(dá)情況九九国产精品久久久久久，發(fā)現(xiàn)RNA干擾轉(zhuǎn)化子中性海藻糖酶mRNA表達(dá)受到抑制，而超表達(dá)轉(zhuǎn)化子中性海藻糖酶mRNA的表達(dá)量是出發(fā)菌株的2倍少妇精品视频一区二区免费看。

Analysis of these mutants revealed that the expression level of the neutral trehalase gene in RNAi mutant (Ma688 strain) was reduced to 42% of which found in wild-type strain and the over-expression mutant (Ma113 strain) showed approximatlely 2-fold higher levels of neutral trehalase mRNA than that found in wild-type control. Then the transformants Ma688 and Ma113 were chosen for closer examination, including trehalose content, storage longevity , thermotol

利用Northern blot 分析這幾個突變菌株中性海藻糖酶基因mRNA 的含量裸体搞黄视频a淫荡，選擇中性海藻糖酶基因超表達(dá)2 倍和中性海藻糖酶基因被抑制到42%的兩個轉(zhuǎn)化菌：Ma113 和Ma688，將其與出發(fā)菌株進(jìn)行分生孢子內(nèi)海藻糖含量的測定日韩寂寞人妻中文字幕，孢子儲藏