After 10 days, 20 days and 30 days for grass carp (Ctenpharyngoden idellus Cuvier et Valencinnes) immunized by being injected Aeromonas hydrophilia, the ANAE (acid α-naphthyl acetate esterase) positive cells ratioes increased, and the same time it was found that the ACP activity increased up too, it showed that the fish bodies were being at immune responding situation and their immnue activities enhanced.

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The soil urease and invertase activity effected by Hg was investigated in different fertility soils. The results show that soil urease activity男女性激烈无遮掩免费观看、maximum reaction velocity (Vmax九九色无码精品网、Vmax/Km) and reaction velocity constant k have remarkably negative correlation with Hg concentration so that these can be used as ecological indexes of soil polluted by Hg, Km changes a little indicating that the reaction mechanism of Hg and urease is nonreversible competitive restraint. Urease of low soil fertility is more damaged by Hg than that of high soil fertility, with severe pollution at Hg concentration of 6.00 and 14.79mg/kg respectively; while invertase is less sensitive to Hg than urease. Cultural time has less influence on the relationship of soil enzyme and Hg concentration.

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But pancreatin is a mixtrure of various enzymes, each enzyme has a low purity and many enzymes have similar molecule structure and physicochemical properties, when extracting one enzyme, it is easy to abandon the otherone as impurity. This is why there are few technoloies of stimutaneous extraction of more than three enzyme from pancreatin and all the technoloies have shortages.

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The enzymatic hydrolyzing technologies of fucoidan from Laminaria japonica using cellulase and pectase were studied.

巖藻聚糖硫酸酯是一種具有多種生物活性的多糖噜噜性爱，對用纖維素酶、果膠酶酶解制備海帶巖藻聚糖硫酸酯的工藝進(jìn)行了研究日日骚一区二区，正交試驗(yàn)結(jié)果表明：纖維素酶99一级屁屁毛片、果膠酶優(yōu)化的酶解工藝參數(shù)為：纖維素酶0.221%、果膠酶0.074%在线免费黄站，溫度50℃黄色网站在线可免费播放国产的，pH值4.5，時間50min午夜理论在线。

The optimum hydrolytic conditions of razor clam for neutral protease were : enzyme concentration 2.4%, hydrolysis temperature 50癈, substrate concentration 1:3, pH 7 and reaction time 6 hrs with the degree of hydrolysis and extraction rate of total nitrogen 42.46% and 83.04% respectively; and foracid protease they were: en/yme concentration 6.0%, hydrolysis temperature 50癈, substrate concentration 1:4, pH3.5 and reaction time 5 hrs with the degree of hydrolysis and extraction rate of total nitrogen 46.37% and 87.94% respectively, the hydrolysate being clear light yellow. For the combination of two kinds of proteases they were: first hydrolyzing with 2.6% f 1 avouryme for 3 hrs, then hydrolyzing with 2.4% neutral protease for 3 hrs with the degree of hydrolysis and extraction rtiie of total nitrogen 4-1.57% and 86.95% respectively.

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Urea-SDS-PAGE showed that no unpurposed propein was found after ConA-sepharose chromatography.2. Identification of alliinaseⅠ, alliinaseⅡ Two different alliinase, named alliinaseⅠand alliinaseⅡ, were found in ConA-sepharose chromatography.

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The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P＜0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P＞0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6％final gel concentration and there were 15～19 bands identified on this condition.

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The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

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Results showed that the activity of soil enzymes was obviously different.Among the different vegetation types,the activity of soil enzymes under Tilia mandshurica forests was the highest except peroxidase,whereas that under shrub-grass was the lowest.The activity of soil urease and alkaline phosphate under shrub was higher than that under the mixed forests.Activity of soil catalase and phosphate under Larix principis-rupprechtii forests was higher than that under Pinus tabulaeformis.

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By TUNEL and ultrastructural observation,the results showed that cadmium toxication caused apoptosis in testes,and adding selenium may decrease the effect. 5.Cadmium can affect the activites of several enzymes in biomembrane, Such as SDH,ALP,Mg2+-ATPase,G-6-P,Ca2+-ATPase and lead to metabolical disorder in tissues.

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