In this paper,the Lactate dehydrogenase, malate dehydrogenase,superoxide dis-mutase of five tissues of Carassius auratus gibelio; Cyprinus carpio Linnaeus; Esox americanus Gmelin; Leuciscus waleckii in Dalai Lake were studied by vertical polypropylene gel electrophoresis and the isozyme composion of four kinds of fishes were obtained. Then the analysis of the expressing specify of 3 kinds of isozyme in different tissues and loci were carried on and the genetic base of isozyme system was discussed.

本實(shí)驗(yàn)采用垂直板聚丙烯酰胺凝膠的電泳方法對(duì)達(dá)賚湖的野生鯉魚(yú)伊人久久久AⅤ老熟妇二区三区、銀鯽啪啪啪精品、狗魚(yú)黄色网欧美、東北雅羅魚(yú)的五種組織臟器(肌肉亚洲午夜九九九九九、心臟、肝臟午夜少妇性私人影院、腦99re视频在线这里、眼睛)的三種同工酶(蘋(píng)果酸脫氫酶、乳酸脫氫酶野花社区大全免费观看3超碰、超氧化物岐化酶)進(jìn)行了初步研究国产精品区在线，獲得了達(dá)賚湖四種魚(yú)類物種的同工酶譜，在此基礎(chǔ)上對(duì)同工酶在不同組織的表達(dá)差異和基因位點(diǎn)進(jìn)行分析日韩一级大日韩女大片一级，探討其同工酶系統(tǒng)的遺傳基礎(chǔ)。

749Mg/ml and 0.778u/ml, respectively. 5. Study on the viscosity changes of reaction solution and analysis of enzymatic hydrolysis products by TLC, HPLC showed that ChiB was an endo-type cleavage enzyme. It decreased viscosity of reaction solution, hydrolyzed β-(1, 4) glucosidic bond of chitosan, acted on GlcN-GlcN, GlcN-GlcNAc and/or GlcNAc-GlcN glucosidic bond. It did not act on GlcNAc-GlcNAc glucosidic bond. Hydrolysis products of chitosan were composed of chitotriose, chitotetraose and chitopentose.

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Methods: Serum alcohol and ALT国产800AV私人在线观看、AST、GGT国语对白露脸刺激自拍、ALP潮喷人人操、CK、LDH三级片黄片日韩、AMY视色视频免费在线观看、LIPA measured before and after beer consuming (4ml/kg) in 17volunteers. Meanwhile, the direct inhibitory effects of beer on the serum enzymes were studied by comparing the serum enzyme activities with or without beer.

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In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

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The experiment showed: 1PER, EST and AMY were polymorphic, meanwhile the isozymic chromatograph could identify the cultivars in this study; 2Two multifoliolate cultivars had specific isozyme loci PER-4, and the level of PER activity of the internal cultivars was higher than the external cultivars; 3 Genetic similarity and euclidean coefficient between the cultivars were 0.826-0.952, 0.500-1.118, between the multifoliolate cultivars were 0.904, 0.500; 4 Multifoliolate cultivares were classified for one type, and others for two other types.

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Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

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By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

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HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

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The β-CA of B.napus was located in chloroplast stroma and had the conservative motif Cys-X(subscript n-His-X2-Cys peculiar to β type carbonic anhydrases. In the secondary structure,α-helix, random coil,β-sheet, and β-turn were 39.88%, 39.27%, 16.31%, and 4.53%, respectively. The three-dimensional structure was constructed by homology modeling. The 3-D alignment of β-CA from B.napus and a monomer of β-CA octamer from pea showed that the 3-D structure of β-CA from B.napus could match well with the monomer. The alignment results Implied that β-CA from B.napus was a monomer of the β-CA octamer from B.napus.

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Comparing with chicken egg-white lysozyme which is widely used in clinic, hLYZ has many advantages: it is a natural protein in human body, so it is safe and harmless when used as a drug; its bioactivity to kill bacterium is three times higher than chicken egg-white lysozyme, and its thermal stability is much higher, too; it also has many other specific important functions which are not related with its catalysis, such as anti-virus and anti-tumor and improving immunization.

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