Apha amylase was difficult to bind with the substrate, ther fore the hydrolysis rate decreased with time.

此外，當酶水解發(fā)生到一定程度在线看片免费人成视频久网不卡，淀粉分子鏈長度變得很短免费A片吃奶玩乳视频。alpha淀粉酶與酶作用物的結(jié)合較難，因此水解速率隨著反應的進行時間下降亚洲最大人成在线视频。

Objective To study the protective effect of coenzyme complex on myocardium under cardiopulmonary bypass in open heart surgery.

目的 觀察輔酶復合物在體外循環(huán)心內(nèi)直視術(shù)中的心肌保護作用无码下体流水性视频。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我們根據(jù)在脲或鹽酸胍存在下的底物反應動力學方程求得游離酶和酶底物二元、三元復合物的微觀動力學常數(shù)18禁黄黄污污网站在线看，結(jié)果表明：①酶-底物二元亚洲已满18岁点击进入久久、三元復合物的失活速度明顯慢于游離酶，說明兩個底物二氫葉酸和NADPH對酶的失活都具有一定程度的保護作用97久久人妻网站；②在脲作用下伊人网免费视频，酶的失活和構(gòu)象變化均為單指數(shù)項過程，而且酶的活力喪失和三級結(jié)構(gòu)變化是同時發(fā)生的免费看黄免费的免费，說明二氫葉酸還原酶的脲變性可能是一個"全或無"的兩態(tài)過程一级牲活片在线观看；③在鹽酸胍作用下，酶的構(gòu)象變化為兩相過程绿茶小视频在线观看，而失活則是單指數(shù)項過程超碰国产在线观看，酶分子構(gòu)象變化的快相速度常數(shù)與失活速度常數(shù)基本一致，因此我們認為二氫葉酸還原酶的鹽酸胍變性過程中存在一個沒有活力国产人成精品免费视频、但仍具備一定空間結(jié)構(gòu)的變性中間體黄色视频三级片AAAAAAAAA。

Chapter 5 covered the quantum chemistry study of the model molecule (410 atoms) of HupA- Acetylcolinesterase complex at HF/4-31G level with point charge model (1929 point charges) to simulate the environment effect on complex active site.

第五章：用HF/4-31G量子化學方法對單分子狀態(tài)石杉堿甲、活化狀態(tài)的石杉堿甲以及石杉堿甲-乙酰膽堿酯酶復合物的模型體系（410原子中文字幕久久精品无码综合网泰国，1929個點電荷）進行了從頭計算研究色欲色欲天天免费视频，指認了對于形成穩(wěn)定復合物貢獻最大的石杉堿甲分子的基團和酶的氨基酸殘基，揭示了活性位點中水分子的重要作用色无码免费视频一区二区。

According to the experiment results of degradation in field, degradation mechanism of the biodegradable mulch film can include four stages: the first, the material surface appeared deficiency while the surface area and interstice increased of material in the action of natural environment and microorganism; the second, along with the surface area and interstice of material increased and the gap strengthened, the chemical chain of the high polymer's principal chain broke as a result of the hydrolysis or the enzymatic effect in the action of hydration, causing the molecular weight and mechanics function descended; the third, the molecular of high polymer split the low polymer after its physical mechanical function descended, the material decomposed the fragments, the mass defect intensified; the fourth, the low polymer of fragments form in the natural environment, the microorganism, the biological enzyme became the smaller fragments, thus absorbed by the phagocytes, or further turn into CO2 and H2O due to hydrolysis.

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Transition metal complexes based on benimidazoles can be used as simulacrum of biology molecular, even more catalytic activity was found in some of them.

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PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

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The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

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An enzyme that catalyzes the removal and transfer of hydrogen from a substrate in an oxidation-reduction reaction.

脫氫酶一種酶，能在氧化還原反應過程中，對脫去作用物中的氫并轉(zhuǎn)化氫起催化作用

Methods The DNA chip was fabricated,on which oligonucleotide bougies′length was 19 base pairs,and the concentration was above 15 μmog/L.

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