The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1无码99riav片、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1久久偷窥一级黄片、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制備SD大鼠肝纖維化動物模型呦呦色色网，連續(xù)灌胃6周亚洲无线码在线一区观看，處死動物取血，檢測血清ALT天天色天天爽、AST一级片免费观看、ALB；ELISA法測定血清透明質(zhì)酸www.色色.com；取肝无码有码日韩制服AV、睥，并計算肝91自拍偷拍第一页、脾指數(shù)日韩人人操人人射，組織切片HE染色后進行組織病理學療效評估；用SP免疫組化法測定肝組織TGF-β1黑人与黑人作爱A级毛片、PPAR-γ及PDGF蛋白表達老熟妇一区二区免费视频；半定量RT-PCR方法檢測肝組織TGF-β1、MMP-1人妻欧美精品片在线97、TIMP-1www。五月天。com、α-SMA、PPAR-γ和iNOSmRNA的表達国内免费的黄色视频在线观看。

The invention discloses a method for measuring seawater chemical oxygen demand by photometry, wherein the seawater exemplar is first pre-processed, then using the basic potassium hypermanganate as reaction system, the potassium permanganate is deacidized quantitatively by organics to produce manganate, using pure water or margin solution as reference ratio, measuring the absorbance an of the manganate at its maximum absorbing wave length 430 nm, drafting the A-COD value operating curve, obtaining the concentration of the organics in the water, i.e.

本發(fā)明公開了一種對海水化學需氧量進行測定的光度測定法两个人的黄色网站，該方法首先將海水樣品進行預處理，再以堿性高錳酸鉀為反應(yīng)體系，這樣海水中的有機物在強堿性介質(zhì)中與過量的高錳酸鉀迅速反應(yīng)亚洲a级黄片免费观看，高錳酸鉀被有機物定量還原后生成錳酸鹽干处女视频，以純水或空白溶液作參比，在錳酸鹽的最大吸收波長430nm處測其吸光度A欧美久久久久精品一区，該吸光度A與海水樣品中的有機物的含量具有正相關(guān)關(guān)系男子把美女尿口亲出水视频。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠隨機分3組：糖尿病組、高蛋氨酸組和葉酸一级大黄片操逼的、維生素B12組亚洲丰满熟女一区二区哦，C57BL/6鼠為對照(C57)，以相應(yīng)飼料喂養(yǎng)4個月后取材国产香蕉网址在线观看AV，測定血糖国产男女猛烈无遮挡免费视频网站、血同型半胱氨酸、葉酸和維生素B12欧美视频.欧美性爱，以PAS色色色黄视频、六氨銀染色法光鏡下及超微電鏡觀察各組DN病變，比較各組腎小球硬化指數(shù)欧美R18在线；以免疫組化和半定量圖像分析腎小球MMP-9蛋白表達性欧洲熟妇色XXXXX，并以RT-PCR檢測腎臟MMP-9 mRNA水平。

Objective To establish the qualitative study methods for Sinapine thiocyanate in Raphanus sativus L.Methods The contents of Sinapine thiocyanate in Raphanus stativus L.was determined by HPLC.

目的對萊菔子中芥子堿硫氰酸鹽進行定量研究方法采用高效液相色譜法對萊菔子中芥子堿硫氰酸鹽進行含量測定亚洲色图中文字幕一区。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分離人胎肝CD34細胞欧美xo视频，培養(yǎng)4d后，蛋白激酶C特異抑制劑chelerythrine chloride(3μmol/L)處理24h日本国产中文字幕，再加入維A酸處理24h5×10^(-7mol/L40岁四川熟女高潮片，無血清培養(yǎng)基培養(yǎng)5d，Western印跡和半定量RT-PCR法分析維A酸處理前后神經(jīng)特異基因表達2019最新在线观看黄色。

HPLC conditions for the separation of α-acid and β-acid in hops were optimized. Besides, cohumulone and humulone + adhumulone, and colupulone and lupulone + adlupulone were quantified to determine HPLC conditions. The precision and the accuracy of HPLC were verified.

對高效液相色譜法分離酒花中α-酸和β-酸的色譜條件進行了優(yōu)化国产污视频网站免费在线观看，并對合葎草酮和葎草酮＋加葎草酮，以及合蛇麻酮和蛇麻酮＋加蛇麻酮進行了定量久久几网站，確定了色譜條件农村一级久久不卡毛片，并對該方法的精密度和準確度進行了驗證在线手机看片国产精品。

He chromatographic conditions for separation and detection of organic acids and inorganic weak acids with ion exclusion chromatography and nonsuppressed conductivity detection are described in this paper.

用非抑制型電導檢測的離子排斥色譜法黄页网站免费观看大全，以對甲苯磺酸為淋洗液，研究了檸檬酸www.whzjsy.com、蘋果酸免费看黄色网站不卡、抗壞血酸、乙醇酸国产高清无码网址、甲酸欧美色色成、乙酸、丙酸及氫氟酸和碳酸的色譜分離及定量檢測條件黄色网站中文字幕。

Results the positive rates and specificity of fluorescence quantitative pcr, acid-fast stain,were100% and 30% respectively.

結(jié)果 熒光定量 pcrwww.日本在线、抗酸染色法檢測結(jié)核分枝桿菌的特異性分別為100%黄色AAA视频、30%。

The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative pcr and acid-fast stain.

對102例培養(yǎng)涂陽的結(jié)核痰液標本和45例非結(jié)核感染者的痰標本欧美综合自拍亚洲综合图自拍，應(yīng)用熒光定量pcr法国产79av、痰涂片抗酸染色法檢測結(jié)核分枝桿菌。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ曰本人的三级视频、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亞砷酸鈉組和砷酸鈉組給予普通飼料和砷溶液一级特a片。10個月后處死小鼠，血清進行肝功能檢測被男人桶添插；蘇木素－伊紅染色部分肝組織亚洲色图日韩无码一区二区；Trizol-酚－氯仿一步法提取小鼠肝組織總RNA，紫外分光光度法測定總RNA濃度及純度免费的色视频综合大全，實時熒光定量PCR法測肝組織中MMP-8免费爽A片在线观看无打码漫画、MMP-2、Col-Ⅰ中文字幕永久更新、Col-Ⅲ mRNA的表達A片在线免费播放不卡，以18S基因作為質(zhì)控。