P 0.05. Total ruminal bacteria, proteolytic and amylolytic bacteria, and protozoa in rumen enumerated by culture method all increased (P 0.05) in the Trial period. Real-time PCR analysis was used to compare Pre-trial and Trial populations of cellulolytic species, including Butyrivibrio fibrisolvens, Ruminococcus flavefaciens and Ruminococcus albus, which were increased by 7.0, 4.5 and 9.6%(P 0.05), respectively. Two starch-fermenting bacteria species, Streptococcus bovis and Ruminobacter amylophilus, were increased by 25.4% and 14.6%(P 0.05), respectively.

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There are two methods to detect and quantitative analysis Neu5Ac, one is high performance liquid chromatography with the use of Aminex87H column, the other one is barbituric acid method.

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In this thesis, two classes nonquaternary Cholinesterase Reactivators (1,2,4-oxadiazole and a -ketothio Hydroximic Acids derivatives ) and some clinical therapy used reactivators added to almost 40 cases have been caculated by using Comparative Molecular Field Analysis,ab initio and molecular mechanism methods. The characteristics of the molecular orbitals and the quantitative structure-activity relationship have been investigated.

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Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

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It is introduced briefly the modification way and result of lignosulphonate as coal-water slurry additive and determination of the adsorption film thicknesses of the LS and modified lignosulphonate on the smooth surface of coal and microscopic slide by the method of elliptical polarization .

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In order to investigate regulation roles of non-esterified fatty acids,β-hydroxybuty-ric acid and glucose in hepatocyte glycometabolism and lipometabolism in dairy cows, effects of NEFA, BHBA and GLU on expression of neonatal calf glucagon receptormRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.

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Several problems in determinating hexanoic acid by colorimetry was analyzed,factors which influence col-or reaction were studied,the resonable colorimetric method was introduced.

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