METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

無菌抽取的骨髓經(jīng)肝素化后国产区123在线，采用密度梯度離心法及貼壁篩選法分離獲得人骨髓間充質干細胞欧美交配电影在线播放，傳至第5代按1× 108 L-1接種于6孔板內，設立2組中文字幕一区欧美，依達拉奉組細胞達50%融合時用含堿性成纖維生長因子日本一区二区三区精品福利、胎牛血清的L-DMEM預誘導24 h，PBS洗滌后再用20 mg/L依達拉奉無血清L-DMEM誘導24 h安全免费黄视频网站；空白對照組始終用含體積分數(shù)為10%胎牛血清的L-DMEM培養(yǎng)亚洲天天综合网在线，不加任何預誘導劑和誘導劑。

In order to clarify the mechanisms of PEMF on osteoporosis, bone marrow cells derived from both femora and tibiae of ovariectomied rats are cultured in vitro to investigate whether osteoclast proliferation is inhibited by PEMF or not.

為了要更進一步探討電磁場刺激抑制骨質流失的內在機轉免费国产a国产片高清网站，因此本研究將以大白鼠之雙側卵巢切除為模式91嫩草国产在线观看免费，取出長骨骨髓細胞做體外培養(yǎng)，藉此探討特定參數(shù)單脈沖電磁場刺激是否經(jīng)由對骨髓中的蝕骨細胞形成精品人妻系列无码专区不卡，產(chǎn)生抑制作用欧美性爱视频免费的，而達到控制骨質流失的目的。

Objective: To verificate culture看黄片在线天堂在线、amplification色色色色色色色色网、induction anddifferentiation of myoblast and Mesenchymal stem cells of themouse in vitro; to transplant the myoblast and MSCs to mdx mice, themodel animal of DMD, by vena caudalis; To observe the growth andfusion of myoblast and MSCs in the host after transplantation.

目的：研究成肌細胞及骨髓間充質干細胞體外培養(yǎng)、擴增高清激情18性爱啊啊在线欧美日韩不卡、誘導分化看黄片.con；利用成肌細胞及骨髓間充質干細胞經(jīng)尾靜脈注射移植治療DMD模型鼠mdx小鼠；觀察移植后的細胞在宿主體內的生長及融合情況www.99热这里有精品；探討基因治療DMD的方法及可行性欧美特级黄色。

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓間充質干細胞進行體外培養(yǎng)擴增后亚洲第一页综合，通過pCDNA3.1質粒將堿性成纖維細胞生長因子基因轉染至生長狀態(tài)良好的骨髓間充質干細胞，并將轉染后的細胞接種于制備好的豬小腸黏膜下層上手机看片国产午夜精品，進行體外聯(lián)合培養(yǎng)一级A片C视频，構建組織工程皮膚。

To investigated the pathogenesis of AA,the rennin activity,angiotensinⅠ and angiotensin Ⅱ concentration in peripheral blood and BM of 22 AA patients were detected by radioimmunnoassay,16 nonhematological disease patients with nomal blood counts and BM picture were used as control,and the differeence between two groups was compared.

為了探討AA的發(fā)病機制黄色网站在线看免费。，運用放射免疫方法檢測22例AA患者骨髓和外周血腎素活性及AngⅠ日韩自娱自拍AV、AngⅡ濃度，并以16例外周血常規(guī)和骨髓檢查正常的患者為對照組国外12小小女网址，比較兩組之間的差異极品美女黄色视频。

47 Cases of clinicopathologic changes in dry tap bone marrow aspirations were found in our hospital.

作者分析該院發(fā)生的47例骨髓穿刺干抽的臨床及病理改變，47例干抽占同期1319例骨髓穿刺的3.6％国产办公室秘书无码精品99视色。

To the best of our knowledge, this is the first study on the global gene expression of MSCs cocultured with periodontal ligament cells. A particular humoral factor released from periodontal ligament cells is suggested to affect differentiation and proliferation in MSCs.

本試驗是第一篇關于骨髓間充質干細胞和牙周膜細胞共培養(yǎng)后基因表達的全面研究免费国产一级A片一片，我們認為來自牙周膜細胞的體液因素將影響骨髓間充質干細胞的增殖和分化。

Objectives 1 To explore the type, distribution, quantity, and clinical significance of auto-antibodies on bone marrow hematopoietic cells in patients with immunorelated pancytopenia; 2 To evaluate the sensitivity of bone marrow mononuclear cell Coombs tests.

目的 (1)了解免疫相關性全血細胞減少癥患者骨髓造血細胞結合的自身抗體的類型91视频guochan、分布国产高清怕怕怕视频在线观看、數(shù)量及臨床意義(2)考察骨髓單個核細胞直接抗人球蛋白試驗(BMMNC-Coombs)的敏感性。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法體外分離培養(yǎng)人骨髓間充質干細胞另类专区另类在线观看视频，傳至第3代以1×108 L-1密度接種至預置蓋玻片的6孔培養(yǎng)板中亚洲综合色图网站，設立3組：成骨誘導組添加原代培養(yǎng)液后，再加入含10-8 mol/L地塞米松日本在线A级黄页网站、10 mmol/L β-磷酸甘油91大神福利导航、50 mg/L維生素C的成骨誘導培養(yǎng)基；成骨+瘦素誘導組添加成骨誘導培養(yǎng)基后人人曰人人曰，再加入50 nmol/L瘦素精品日韩精品手机在线观看AV；空白對照組僅加入含體積分數(shù)為10%胎牛血清的LG-DMEM培養(yǎng)液。

Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+ selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined.

常規(guī)富集人骨髓CD34+及AC133+細胞国产美女裸精品视频，應用本研究組設計并已經(jīng)證實的細胞因子組合方式亚洲丝袜脚交，對人骨髓CD34+及AC133+富集細胞進行體外對照培養(yǎng)，分別觀察CD34+和AC133+富集細胞的擴增情況最新超碰在线；應用甲基纖維素半固體培養(yǎng)法操逼欧美视频，觀察不同組別培養(yǎng)7、14国产AV自拍网站导航、21d CD34+和AC133+富集細胞的集落形成情況及細胞凋亡率可以看的毛片网址。