With an orthogonal experiment design , the telomerase inhibitors [azidothymidine , AZT 300 mg/ and lamivudine 150 mg/, per os , bid ,×2 weeks ] and X2irradiation [ total dose 10 Gy (2 Gy ×5) in 1 week ] were used to treat BALB/ c mice carrying breast cancer MA782 for evaluating the influence on peripheral blood cells , bone marrow nucleated cells and telomerase activity.

為了探討端粒酶抑制劑聯(lián)合X 線照射對荷瘤小鼠的骨髓抑制作用大秀免费人成网站在线视频，采用正交實驗設(shè)計，應(yīng)用端粒酶抑制劑[AZT 300 mg/， lamivudine 150 mg/ ]聯(lián)合X 線照射(2 Gy ×5次/ 1 周)處理移植性乳腺癌(MA782 )小鼠，觀察其對骨髓有核細(xì)胞欧美系列一级二级在线观看、外周血像及骨髓端粒酶活性的影響。

Proliferation of fibroblastic cells in bone marrow,caus in g anemia and sometimes enlargement of the spleen and liver.

骨髓纖維化欧美一级精品视频，骨髓纖維變性在骨髓中成纖維細(xì)胞的擴(kuò)散黄色真人免费网站，常引起貧血癥，而且有時也會導(dǎo)致脾和肝的增大

The bone marrow and spleen of the mice in both RT and HHE groups were examined at 1, 3, 5, 9 and 15 days after irradiation for bone marrow cellularity, DNA content in the bone marrow cells, and spleen/body weight ratio.

照后1www.色色色网、3、5自拍偷自拍亚洲精品偷一、9点击进入a在线影院、15d，分別取兩組小鼠的骨髓和脾臟欧美一级AAAAA特黄片免费看，獲取骨髓有核細(xì)胞數(shù)色色碰精品、骨髓細(xì)胞DNA、脾/體質(zhì)量比實驗指標(biāo)日韩自拍中文在线视频观看。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基質(zhì)細(xì)胞在體外培養(yǎng)條件下的成骨能力及生物學(xué)特性欧美黄色视频第一次，探討簡便易行的成骨細(xì)胞體外培養(yǎng)方法，為組織工程選擇理想的種子細(xì)胞來源黄网址免费。方法：取新西蘭白兔骨髓液經(jīng)離心后得骨髓單個核細(xì)胞日韩美乳精品无码福利视频，以1× 106／ml的細(xì)胞濃度進(jìn)行培養(yǎng)，經(jīng)傳代培養(yǎng)www.krttz.com，選擇條件培養(yǎng)液培養(yǎng)(1640完全培養(yǎng)液中加入地塞米松10-8Smol／l亚洲日本无吗在线视频，β-甘油磷酸鈉10mmol／l，VitC 50μg／ml)作為骨髓基質(zhì)細(xì)胞增殖和成骨的調(diào)節(jié)因子A片视频免费观看的网址，通過倒置顯微鏡欧美日韩亚洲综合视频、HE染色、掃描電鏡免费观看黄色国产一级视频、堿磷酸酶(alkaline phosphatase无码专区免费大全在线观看，ALP)測定曰批视频免费看10分钟、Ⅰ型膠原免疫組化染色和四環(huán)素?zé)晒忖}染色等手段對獲得的細(xì)胞進(jìn)行生物學(xué)特性研究。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

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Study Design. Two groups of 6 rats received dorsolateral funiculotomies followed by direct injection of bone marrow stromal cells or mono-nuclear fraction of bone marrow.

研究設(shè)計兩個實驗組的6只大鼠收到骨髓，隨后直接注入骨髓基質(zhì)細(xì)胞或單核骨髓細(xì)胞淫AV片。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

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objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

選擇12例尿毒癥腎性貧血未透析患者為試驗組，另選正常健康體檢者12例為正常對照組日韩午夜不卡免费毛片，將實驗組操欧美老妇屄、正常對照組血清分別稀釋成不同終濃度，采用甲基纖維素細(xì)胞培養(yǎng)的方法對小鼠骨髓細(xì)胞進(jìn)行培養(yǎng)日韩成人国语在线视频播放网站，對比不同濃度血清組紅系集落形成單位乐播网久久久精品影院、紅系爆式形成單位的集落數(shù)。結(jié)果在實驗組中欧美性爱蜜腚，與血清濃度為0的濃度組相比逼逼爱插插毛片，與各濃度組血清共同培養(yǎng)的小鼠骨髓cfu-e和bfu-e的集落數(shù)均明顯減少，且隨血清濃度的增加黄色视频污免费网站，小鼠骨髓cfu-e和bfu-e的集落數(shù)逐漸減少成年人三级片，各濃度組間差異有顯著性（p＜0.05）。結(jié)論尿毒癥患者血清抑制小鼠骨髓紅系祖細(xì)胞集落形成黄色视频在线免费，隨患者血清濃度的增加夜夜澡人模人人添人人看，抑制作用增強(qiáng)，具有劑量依賴性亚洲欧美日韩一级特黄在线。

To observe the survival and migration of Bone Marrow Mesenchymal Stem Cells derived from Green Fluoresent Protein Transgenic mice and the change on expression of Fas and Fasl apoptosis factors at different times after transplantation GFP-MSCs via caudal vien to treat contused spinal cord injuryed adult SD rats modal ,and to provide experimental evidence for the reparing function and mechanism which MSCs treat spinal cord injury.

通過靜脈移植綠色熒光蛋白轉(zhuǎn)基因小鼠骨髓間充質(zhì)干細(xì)胞治療大鼠脊髓挫傷模型福利视频免费，觀察骨髓間充質(zhì)干細(xì)胞存活遷移情況及凋亡因子Fas和Fas1在移植后不同時點的變化情況，為骨髓間充質(zhì)干細(xì)胞治療脊髓損傷及機(jī)理提供實驗室依據(jù)曰曰人人公开免费公开视频。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

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